Human MCTS1 Knockout Cell Line-HCT116

Name: Human MCTS1 Knockout Cell Line-HCT116
SKU: CSC-RT0051
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0051

Target Gene : MCTS1 Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0051
Description	HCT116-MCTS1 (-/Y) is a cell line with knockout of X-linked MCTS1 allele in male parental cells
Target Gene	MCTS1
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MCTS1 malignant T cell amplified sequence 1 [ Homo sapiens ]
Gene Symbol	MCTS1
Synonyms	MCTS1; malignant T cell amplified sequence 1; malignant T-cell-amplified sequence 1; MCT 1; multiple copies T-cell malignancies; malignant T cell-amplified sequence 1; MCT1; MCT-1; FLJ39637;
Gene ID	28985
Uni Prot ID	Q9ULC4
m RNA Refseq	BC001013
Chromosome Location	Xq24
Function	RNA binding;
MIM	300587

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Simple process

As a research scientist, I always strive for efficient experimental methods. This brand's MCTS1 knockout cell line not only simplify the process, but also produce amazing results, saving me time and energy.

United States

2020-01-12

Reliable quality

This brand's MCTS1 knockout cell line not only offer reliable quality, but are also very reasonably priced. They are truly a great value!

United States

2021-04-11

Simple steps

Compared to other brands, I prefer using this brand's MCTS1 knockout cell line. They are easy to use and provide stable experimental data.

United States

2021-11-21

Consistent results

Before using this brand's MCTS1 knockout cell line, I struggled with inaccurate and fluctuating experimental data. However, since using their cell lines, my experimental results have been consistently stable and satisfying.

United States

2022-01-31

Continue to use

I have used this brand's MCTS1 knockout cell line multiple times and have been pleasantly surprised every time. It's a truly fantastic product that I will continue to use.

United States

2022-08-05

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Human MCTS1 Knockout Cell Line-HCT116

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Simple process

Reliable quality

Simple steps

Consistent results

Continue to use

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