Cat.No. : CSC-RT0068
Host Cell: HCT116 Target Gene: KDM5A
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0068 |
| Description | HCT116 -KDM5A (-/-) is a cell line with a homozygous knockout of human KDM5A |
| Target Gene | KDM5A |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I have used other gene knockout reagents in the past period of time, but Human KDM5A (-/-) Knockout Cell Line-HCT116 is my go-to. The knockdown efficiency is unbeatable.
The knockdown efficiency of Human KDM5A (-/-) Knockout Cell Line-HCT116 is excellent, and the product is very user-friendly. It has made my research much more efficient.
I have been using Human KDM5A (-/-) Knockout Cell Line-HCT116 for a few experiments now, and I was extremely impressed with the results of the experiment. I would definitely recommend it to others.
Human KDM5A (-/-) Knockout Cell Line-HCT116 is a rescuers for my research. It is highly effective and saves me a lot of time.
I was hesitant to try Human KDM5A (-/-) Knockout Cell Line-HCT116 at first, but I am so glad I did. The knockdown efficiency is incredible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
