Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0559
Target Gene : CLU Host Cell : HeLa
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0559 |
| Description | A stable cell line with a homozygous knockout of human CLU using CRISPR/Cas9. |
| Target Gene | CLU |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Storage | Liquid nitrogen |
| Shipping | Dry ice package |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CLU |
| GeneID | 1191 |
A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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The Human CLU Knockout Cell Line-HeLa has been a crucial addition to our research efforts. The cells are of high quality and the knockout efficiency is exceptional. The detailed documentation provided made it easy for us to incorporate this cell line into our experiments.
The Human CLU Knockout Cell Line-HeLa has truly revolutionized our research. It has enabled us to study the function of CLU in a more detailed manner than ever before, leading to new insights and discoveries. We are grateful for this innovative product.
The quality of the Human CLU Knockout Cell Line-HeLa is remarkable. We have noticed minimal cell death and excellent cell viability throughout our experiments. This has allowed us to obtain accurate and reliable results, which has been crucial for our research.
Integrating the Human CLU Knockout Cell Line-HeLa into our existing experimental workflow has been straightforward. The documentation provided made it easy to understand the cell line's properties and how to best utilize it in our experiments.
We appreciate the effort the company has put into ensuring the ethical sourcing of the Human CLU Knockout Cell Line-HeLa. This is of utmost importance to us, and it's reassuring to know that the cells we are using have been obtained through ethical means
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