Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT2120
Target Gene : ATF3 Host Cell : A549
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT2120 |
| Description | This cell is a stable cell line with a homozygous knockout of human ATF3 using CRISPR/Cas9. |
| Target Gene | ATF3 |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Storage | Liquid nirtogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | ATF3 activating transcription factor 3 [ Homo sapiens ] |
| Gene Symbol | ATF3 |
| Synonyms | ATF3; activating transcription factor 3; cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; FLJ41705; |
| GeneID | 467 |
| Uni ProtID | P18847 |
| mRNA Refseq | BC006322 |
| Chromosome Location | 1q32.3 |
| Function | identical protein binding; protein binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; |
| Pathway | ATF-2 transcription factor network, organism-specific biosystem; Activation of Genes by ATF4, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Disease, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; |
| MIM | 603148 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
In the presence of specific antibodies, Human ATF3 KO cells do not produce a signal in the validation result, whereas wild-type cell lines may produce a specific protein signal, so they can be a good standard for antibody validation to determine the specificity of antibodies.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.