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Cat.No. : CSC-RT0164
Host Cell: 293T Target Gene: GPAA1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0164 |
| Description | 293T-GPAA1 (-/-) is a stable cell line with a homozygous knockout of human GPAA1 |
| Background | Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008] |
| Target Gene | GPAA1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
GPAA1 Knockout Cell Line-293T has significantly advanced my genetic research, providing a reliable and effective tool for precise gene manipulation and unraveling the mysteries of gene function.
France
04/11/2023
I am impressed by the high knockout efficiency of GPAA1 Knockout Cell Line-293T, which consistently delivers successful gene knockouts with remarkable precision.
United States
05/09/2023
The simple protocol and clear instructions provided with GPAA1 Knockout Cell Line-293T have streamlined my experimental process, allowing me to focus more on data analysis and interpretation.
United States
08/23/2020
The reproducibility and reliability of the results obtained using GPAA1 Knockout Cell Line-293T have strengthened the credibility of my research, providing robust evidence to support my scientific conclusions.
United States
05/12/2021
I appreciate the exceptional support and assistance provided by the manufacturer, who has been readily available to address any questions or concerns during my gene knockout experiments.
United States
08/15/2021
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