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Cat.No. : CSC-RT0120
Host Cell: Hela Target Gene: FHL1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0120 |
| Description | Hela-FHL1 cell line is a stable cell line with a homozygous knockout of FHL1 |
| Target Gene | FHL1 |
| Host Cell | Hela |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: DMEM supplemented with 10% fetal bovine serum.
It is not required to add the selection antibiotics when culturing the KO cells.
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
FHL1 Knockout Cell Line-Hela has opened up new possibilities for my research, allowing me to explore gene functions and their impact with precision.
Germany
08/09/2023
I was pleasantly surprised by the time efficiency of FHL1 Knockout Cell Line-Hela, enabling me to complete my experiments in a timely manner.
United States
08/23/2020
The high success rate of gene knockout achieved with FHL1 Knockout Cell Line-Hela has boosted my confidence in the reliability of my experimental outcomes.
United States
05/12/2021
The consistent and reproducible results obtained using FHL1 Knockout Cell Line-Hela have strengthened the validity of my research findings.
France
08/15/2021
The compatibility of FHL1 Knockout Cell Line-Hela with various cell types and organisms expands its versatility and applicability in different research areas.
United States
10/09/2021
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