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FAM129B Knockout Cell Line-293T

FAM129B Knockout Cell Line-293T

Cat.No. :  CSC-RT0206

Host Cell:  293T Target Gene:  FAM129B

Size:  1x10^6 cells/vial, 1mL Validation:  Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0206
Description 293T-FAM129B (-/-) is a stable cell line with a homozygous knockout of human FAM129B
Target Gene FAM129B
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Accelerated progress

FAM129B Knockout Cell Line-293T has significantly accelerated my research progress, enabling me to unravel the intricate mechanisms of gene function with precision.

United States

07/19/2022

Reproducibility

The reproducibility of results obtained with FAM129B Knockout Cell Line-293T has strengthened the robustness and impact of my scientific findings.

France

11/03/2022

New possibilities

FAM129B Knockout Cell Line-293T has transformed the landscape of genetic research, unlocking new possibilities and driving scientific advancements.

United States

02/23/2023

Implications

FAM129B Knockout Cell Line-293T's contribution to advancing our understanding of gene function and its implications in various fields is truly remarkable.

United States

04/11/2023

Elevated the qualit

By choosing FAM129B Knockout Cell Line-293T, I have elevated the quality and significance of my research, paving the way for groundbreaking discoveries.

United States

08/23/2020

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