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Cat.No. : CSC-RT0098
Host Cell: RAW 264.7 Target Gene: Dguok
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0098 |
| Description | raw264.7-Dguok cell line is a stable cell line with a homozygous knockout of Dguok |
| Target Gene | Dguok |
| Host Cell | RAW 264.7 |
| Host Cell Species | Mus musculus (Mouse) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Dguok Knockout Cell Line-raw264.7 has exceeded my expectations in every aspect. Its high knockout efficiency, ease of use, and consistent results have elevated the quality and impact of my research.
United States
01/27/2022
I am thrilled with the performance of Dguok Knockout Cell Line-raw264.7. It has enabled me to generate reliable and reproducible knockout models, providing valuable insights into gene function and disease mechanisms.
United States
07/19/2022
Dguok Knockout Cell Line-raw264.7 has simplified the gene editing process for me. Its intuitive workflow and outstanding knockout efficiency have allowed me to focus more on data analysis and interpretation.
United States
11/03/2022
I have tried several gene knockout reagents, but Dguok Knockout Cell Line-raw264.7 stands out for its exceptional performance. Its high success rate and consistency have made it an indispensable tool in my research arsenal.
United States
02/23/2023
I confidently recommend Dguok Knockout Cell Line-raw264.7 to my colleagues. Its efficiency, reliability, and ease of use have transformed the way I approach gene editing experiments, leading to significant advancements in my field.
United States
04/11/2023
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