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Dguok Knockout Cell Line - RAW 264.7

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0098

Target Gene :   Dguok Host Cell :   RAW264.7

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0098
Description raw264.7-Dguok cell line is a stable cell line with a homozygous knockout of Dguok
Target Gene Dguok
Host Cell RAW264.7
Host Cell Species Mus musculus (Mouse)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene DGUOK
Background In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Exceeded my expectations

Dguok Knockout Cell Line-raw264.7 has exceeded my expectations in every aspect. Its high knockout efficiency, ease of use, and consistent results have elevated the quality and impact of my research.

United States

Reliable and reproducible

I am thrilled with the performance of Dguok Knockout Cell Line-raw264.7. It has enabled me to generate reliable and reproducible knockout models, providing valuable insights into gene function and disease mechanisms.

United States

Simplified progress

Dguok Knockout Cell Line-raw264.7 has simplified the gene editing process for me. Its intuitive workflow and outstanding knockout efficiency have allowed me to focus more on data analysis and interpretation.

United States

High success rate

I have tried several gene knockout reagents, but Dguok Knockout Cell Line-raw264.7 stands out for its exceptional performance. Its high success rate and consistency have made it an indispensable tool in my research arsenal.

United States

Efficiency, reliability, and ease of use

I confidently recommend Dguok Knockout Cell Line-raw264.7 to my colleagues. Its efficiency, reliability, and ease of use have transformed the way I approach gene editing experiments, leading to significant advancements in my field.

United States

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