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COA1 Knockout Cell Line-293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0160

Target Gene :   COA1 Host Cell :   293T

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0160
Description 293T-COA1 (-/-) is a stable cell line with a homozygous knockout of human COA1
Target Gene COA1
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Invaluable tool

COA1 Knockout Cell Line-293T has proven to be an invaluable tool in my genetic research, enabling precise and targeted gene modifications with remarkable efficiency.

France

Accuracy and reliability

I am impressed by the high success rate of gene knockouts achieved with COA1 Knockout Cell Line-293T, allowing me to investigate the functions of specific genes with accuracy and reliability.

United States

Straightforward workflow

The easy and convenient protocol and straightforward workflow of COA1 Knockout Cell Line-293T have simplified the experimental process, making it accessible to researchers with varying levels of expertise.

United States

Consistent and reproducible results

The reliable results obtained using COA1 Knockout Cell Line-293T have bolstered the validity of my research findings and enhanced the overall scientific rigor of my work.

United States

Responsive and helpful

I am highly grateful for the exceptional customer support provided by the manufacturer, who has been responsive and helpful in addressing any queries or concerns I encountered during my gene knockout experiments.

United States

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