Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0234
Target Gene : CLPP Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0234 |
| Description | 293T-CLPP (-/-) is a stable cell line with a homozygous knockout of human CLPP |
| Background | The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| Target Gene | CLPP |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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As a researcher who has had the opportunity to work with CLPP Knockout Cell Line-293T, I can confidently say that it has been a valuable asset in my laboratory endeavors.
CLPP Knockout Cell Line-293T's consistent performance and high efficiency have allowed me to achieve precise gene editing, enabling me to explore gene functions with reliability and accuracy.
I appreciate the user-friendly protocol and clear instructions, which have made the experimental process straightforward and saved me time in the lab.
CLPP Knockout Cell Line-293T has delivered reliable and reproducible results, providing a strong foundation for my research findings and contributing to the credibility of my scientific publications.
The manufacturer's responsive and knowledgeable customer support has been instrumental in addressing any questions or challenges, ensuring a smooth and successful research journey.
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