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Cat.No. : CSC-RT0224
Host Cell: 293T Target Gene: CDC42
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0224 |
| Description | 293T-CDC42 (-/-) is a stable cell line with a homozygous knockout of human CDC42 |
| Target Gene | CDC42 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Beyond its scientific impact, CDC42 Knockout Cell Line-293T has shaped my perspective as a researcher, instilling in me a deep appreciation for the intricacies of genetic mechanisms.
United Kingdom
10/09/2021
As I collaborate with fellow scientists who also use CDC42 Knockout Cell Line-293T, I am inspired by the diverse range of research projects it has facilitated, leading to a vibrant exchange of ideas.
United States
01/27/2022
The profound satisfaction of achieving successful gene knockouts and the thrill of uncovering new insights motivate me to continue pushing the boundaries of genetic research.
United States
07/19/2022
With each step forward, I am grateful for the support of CDC42 Knockout Cell Line-293T, which has transformed my journey as a researcher into an exhilarating quest for knowledge.
United States
11/03/2022
To the developers and manufacturers of CDC42 Knockout Cell Line-293T, I extend my heartfelt gratitude for providing such a remarkable tool that has undoubtedly left a lasting mark on my scientific career.
United States
02/23/2023
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