Cat.No. : CSC-RT0273
Host Cell: 293T Target Gene: BCAP31
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0273 |
| Description | 293T-BCAP31 (-/-) is a stable cell line with a homozygous knockout of human BCAP31 |
| Background | This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012] |
| Target Gene | BCAP31 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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As I look back on my research journey with BCAP31 Knockout Cell Line-293T, I am reminded of the impact it has had on shaping not only my scientific work but also my approach to problem-solving and critical thinking.
The ability to selectively disrupt gene function has opened doors to novel research avenues, allowing me to investigate gene interactions and signaling pathways that were previously inaccessible.
The reproducibility of results obtained with BCAP31 Knockout Cell Line-293T has bolstered the credibility of my research, enhancing the robustness of my findings and contributing to the wider body of scientific knowledge.
In my collaborations with industry partners, BCAP31 Knockout Cell Line-293T has become a key asset in the development of innovative biotechnological solutions, with potential applications in fields such as biopharmaceuticals and biofuels.
I am inspired by the countless possibilities that lie ahead, as BCAP31 Knockout Cell Line-293T continues to expand our understanding of the genetic landscape, leading to discoveries that may reshape the future of healthcare, agriculture, and environmental conservation.
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