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Cat.No. : CSC-RT0130
Host Cell: LLC-MK2 Target Gene: APOE
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0130 |
| Description | LLC-MK2-ApoE cell line is a stable cell line with a homozygous knockout of ApoE |
| Target Gene | APOE |
| Host Cell | LLC-MK2 |
| Host Cell Species | Macaca mulatta (Rhesus macaque) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
ApoE Knockout Cell Line-LLC-MK2 has been a game-changer for my research, providing a powerful tool to investigate the functions of specific genes with high efficiency and accuracy.
United States
11/03/2022
I appreciate the consistent and reproducible results I have obtained with ApoE Knockout Cell Line-LLC-MK2, ensuring the reliability of my experimental outcomes.
United States
02/23/2023
The ease of use and reliability of ApoE Knockout Cell Line-LLC-MK2 have made it a valuable asset in my laboratory, allowing me to focus more on the scientific aspects of my research.
United States
04/11/2023
I am impressed with the versatility of ApoE Knockout Cell Line-LLC-MK2, as it has demonstrated successful knockouts in a variety of cell lines and experimental models.
United Kingdom
05/09/2023
The outstanding performance of ApoE Knockout Cell Line-LLC-MK2 has significantly accelerated my research progress,enabling me to uncover new insights and generate impactful discoveries.
United States
05/31/2022
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