Robust Tn5 Transposase is a hyperactive form of Tn5 transposase. This enzyme can be used to randomly insert Tn5 transposon into target DNA. Robust Tn5 Transposase recognizes inside end sequences, outside end sequences and mosaic end sequences of Tn5 transposon.
An E.coli strain that carries Robust Tn5 transposase gene.
• in vitro transgenic experiment
• Construction of random library for second-generation sequencing
One unit Tn5 transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hourat 37°C.