Creative biogene
SHARE THIS
facebook twitterlinkedingoogle plusStumbleUpon blog


Subtractive cDNA Library Construction Service

email info@creative-biogene.com Tel: 1-631-626-9181 Fax: 1-631-614-7828
Creative Biogene is a leading biotechnology company which can provide high quality subtractive cDNA library construction services for customers worldwide. Creative Biogene’s proprietary, self-subtraction/ tissue-tissue subtraction techniques and highly experienced staffs are able to irrelevant abundant sequences 10-100 folds, making it easier to find rare or low-expressed target genes. Besides, with years of experience in the cDNA library construction field, Creative Biogene can provide the most affordable subtractive cDNA library construction services with fastest turnaround time to satisfy your downstream needs.


Schematic diagram of the subtractive cDNA library construction [1]


A subtractive cDNA library is a collection of cDNA clones which is rare and likely to be poorly expressed. Subtractive cDNA libraries are produced using a proprietary technique which relies on the removal of dsDNA formed by hybridization between a control and test sample, thus eliminating cDNAs of similar abundance and retaining the transcripts which are differentially expressed or variable in sequence. As a leading supplier in cDNA library construction services, Creative Biogene can provide you with high-quality subtractive cDNA library construction services to ensure your satisfaction in a timely and professional manner.


Applications:

  1. Cloning new genes.
  2. Analysis of gene expression profiles.


Features:

  1. Large numbers of subtracted clones.
  2. Low vector background and high percentage of recombinants.
  3. The libraries can be made directionally and in the vector of your choice.
  4. Competitive prices.
  5. Fast turnaround time.



Creative Biogene offers subtractive cDNA library construction services for your scientific research as follows:
  1. Extraction RNA from a variety of samples.
  2. Synthesis full-length cDNA
  3. Hybridization and eliminating cDNAs of similar abundance.
  4. Directional cloning the target cDNAs into any vector.
  5. Production of a library with at least 106 cfu.
  6. Verification of library quality by randomly picking clones and determining insert sizes by restriction digest or DNA sequencing.


References:
[1] Carninci P, Shibata Y, Hayatsu N, et al. Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes. Genome Res. 2000, 10(10):1617-30.
[2] Klickstein LB. Production of a subtracted cDNA library. Curr Protoc Mol Biol. 2001, Chapter 25.

Quick Inquiry

Please input "biogene" as verification code.
  Order Info:Subtractive cDNA Library Construction Service  order info
Services
Contact us to order
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email:info@creative-biogene.com
Europe
Tel: 44-207-048-3343

Email:info@creative-biogene.com
CBpromise

24x7 CUSTOMER SERVICE

CONTACT US TO ORDER


CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-048-3343

Terms & Conditions | Privacy Policy | Sitemap | FAQ | © 2010-2017 Creative Biogene. All Rights Reserved.
-->