Creative Biogene is the leading company offering the first-class ion channel binding assay services. As a pioneer in this field, based on years of research experience, Creative Biogene provides services to detect the binding affinity of a compound to your target ion channel.
Creative Biogene utilizes the gold standard filtration method to perform the highest assay robustness. We offer radioligand binding assays across a wide range of ion channel families, including sodium, potassium, and calcium, and so on. By radioligand displacement assays, Creative Biogene can help you determine the interaction and calculate a compound's affinity for a channel of interest.
High-throughput ion channel binding assays have been configured for filter binding assays, scintillation proximity assays, and fluorescence polarization assays. Ligand binding assays can detect compounds that compete with ligands for binding to a specific site. Given its low cost and high throughput, binding assays have been developed for many voltage-gated and ligand-gated ion channels. Ligand binding assays require previous information of the target binding sites and the formation of a radioligand which is specific to those binding sites. Activity of the interest compound is indicated by the displacement of the labeled ligand (Haibo, et al. 2016).
Fig.1 Equilibrium binding affinity of JNJ-54232334 (Brian, et al. 2015)
Consequently, conventional instrumentation can be used, in which throughput represents its major strength. Because the method only discovers compounds affecting radioligand binding, it misses allosteric modulators of ion channels. Binding assays identify affinity data but cannot identify the functional change of ion channels. Therefore, by combining with functional assays, Creative Biogene offers ion channel binding studies that can provide an orthogonal approach to creating a full profile of your compound's in vitro pharmacology.
Fig.2 The structure of Ion channel (Sharan et al. 2012)
Advantages of Ion Channel binding assay services in Creative Biogene:
With years of experience and expertise in this field, Creative Biogene can provide you the best services and highest quality results for your ion channel binding assays. Our talented scientists will work closely with you to effectively detective your compound binding affinity. Creative Biogene is confident in speeding your project progress and achieving ideal research goals with the most competitive price.
If you have any special requirements in Ion channel binding assays, please feel free to contact us at firstname.lastname@example.org or 1-631-626-9181. We are looking forward to working together with your attractive projects.
1.Brian, L., Michael, K.A., Qi, W., Lawrence, F., Maarten, V., Willy, V., Pieter, H., Nicholas, I.C., Timothy, W.L., Pascal, B., Michael, A.L., Anindya, B. (2015) ‘A novelradioligandfortheATP-gatedionchannelP2X7:[3H] JNJ-54232334’, European Journal of Pharmacology,765, 551–559
2.Sharan, K. B., Alan, D. B., Peter, J. C., Kiyoyuki, O., Robert, M. O., David, C. P., Benjamin, S., Sarah, E. S., Edward, B. S., R. Ian, S., Nigel, A. S. (2012) ‘Ion Channels as Therapeutic Targets: A Drug Discovery Perspective’, J. Med. Chem., 56, 593?624
3.Haibo, Y., Min, L., Weiping, W., Xiaoliang W. (2016) ‘High throughput screening technologies for ion channels’, Acta Pharmacologica Sinica, 37, 34-43