Creative biogene
SHARE THIS
facebook twitterlinkedingoogle plusStumbleUpon blog


Stable Knock-in Cell Line Generation

email info@creative-biogene.com Tel: 1-631-626-9181 Fax: 1-631-614-7828

Brief Introduction


CRISPR/Cas9-induced site-specific DNA double-strand breaks (DSBs) can be repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) pathways. Extensive efforts have been made to knock-in exogenous DNA to a specific genomic locus via the HDR pathway in mammalian cells.

Creative Biogene, as a leader in cell engineering, provides reliable, affordable, and fast knock-in cell line generation service, which includes gRNA/donor DNA construction, PCR sequencing, and engineered cell line generation. We have experience working with a wide variety of cell backgrounds ranging from easy-to-transfect cell lines (like HEK2993, HCT116 or HeLa) to hard-to-transfect cell lines (like iPSCs, fibroblasts). Creative Biogene produces knock-in cell lines and performs quality control testing in one step to help ensure that the cell line meets your requirements.

Features

  1. One stop service- We provide full service from gRNA/donor DNA construction to engineered cell line generation.
  2. Reliable- All stable cell lines provided by Creative Biogene are guaranteed free of mycoplasma contamination.
  3. Custom services- We provide custom services tailor made based on your request.
  4. A wide variety of cell type- We have worked with everything from HEK293 to iPSCs.
  5. Kinds of genome modifications- Introduce/correct point mutations, safe harbor KI, SNP edit and insert a tag/fluorescent protein into the target gene.

Process

  1. gRNA/donor DNA construction
  2. Co-transfect into cells of interest
  3. Validation of custom CRISPR efficacy
  4. Screening of positive cell clones
  5. Quality control analysis of stable cell line
  6. Delivery of stable cell line

Related Protocols:
How to deisgn sgRNA for your CRISPR
CRISPR Plasmid Transfection
T7E1 Assay Protocol

FAQ:
Stable Knock-in Cell Line Generation

Quick Inquiry

Please input "biogene" as verification code.
  Order Info: Stable Knock-in Cell Line Generation  order info
Services
Contact us to order
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
CBpromise

24x7 CUSTOMER SERVICE

CONTACT US TO ORDER


CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

Terms & Conditions | Privacy Policy | Sitemap | FAQ | © 2010-2017 Creative Biogene. All Rights Reserved.
-->