Molecular biology techniques are common methods used in biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA and RNA.
Gene synthesis, also known as DNA printing, is a common method in synthetic biology used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs.
Another most basic technique of molecular biology to study protein function is gene cloning. In this technique, target genes are cloned (using PCR and/or restriction enzymes) into vectors as expression plasmids to assemble recombinant DNA molecules and to direct their replication within host organisms.
Creative Biogene provides comprehensive molecular biology services, especially gene synthesis and cloning of any sequence on any scale, as well as oligonucleotides synthesis, DNA methylation and plasmid DNA production services. As the business leader, we are proud to provide flexible and reliable molecular biology services to accelerate your research.
Our state-of-the-art labs are staffed by some of the best trained and most experienced subject matter experts in the field. Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.