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BAC Library Construction Service

email info@creative-biogene.com Tel: 1-631-626-9181 Fax: 1-631-614-7828
Creative Biogene is a leading biotechnology company specializing in custom BAC library construction services. Creative Biogene’s advanced technologies and highly experienced staffs are able to provide you custom BAC libraries with average insert sizes between 115 kb ~ 180 kb, and optimize protocols to be successful where others have failed. Creative Biogene’s goal is to provide you with the most affordable, high-quality BAC library construction services to ensure your satisfaction in a timely and professional manner.
Bacterial Artificial Chromosome (BAC) acts as a vector to artificially carry DNA into the cell of a bacterium, such as Escherichia coli, to make a BAC clone. In general, BAC clones can carry inserts of DNA up to 200,000 base pairs long. Recently, BAC is used to create BAC libraries which are preferable for genome sequencing, physical maps constructing, and gene cloning. As a leading supplier in BAC library construction services, Creative Biogene is committed to providing you the most affordable BAC library construction services with fastest turnaround time to satisfy your downstream needs.


Creative Biogene is able to provide the following BAC library construction services for your scientific research:
Arrayed/Non-arrayed BAC Library
An arrayed or non-arrayed BAC library is the preferred choice for researchers wishing to screen using hybridization techniques. An arrayed library is also the only template that will do for whole genome sequencing or physical mapping.
Screened/Pooled BAC Library
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive.


Applications:

  1. Study the genome sequence of a particular gene
  2. Determine the complete genome sequence of a given organism.
  3. Construct physical maps


Features:

  1. Large numbers of primary clones for better coverage of whole genome.
  2. Low vector background and high percentage of recombinants.
  3. Large insert sizes.
  4. Competitive prices.
  5. Fast turnaround time.


References:
[1] O'Connor M, Peifer M, Bender W. Construction of large DNA segments in Escherichia coli. Science (Washington), 1989, 244(4910): 1307-1312.
[2] Shizuya H, Kouros-Mehr H. The development and applications of the bacterial artificial chromosome cloning system. The Keio journal of medicine, 2001, 50(1): 26-30.
[3] Osoegawa K, de Jong P J. BAC library construction. Bacterial Artificial Chromosomes: Volume 1 Library Construction, Physical Mapping, and Sequencing, 2004: 1-46.

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email:info@creative-biogene.com
Europe
Tel: 44-207-048-3343

Email:info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-048-3343

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