Exon trapping and targeted regions trapping are commonly used in the second generation sequencing methods, which require a one-time synthesis of tens to millions of primers. If use the traditional method for synthesis of primers, the cost is very high.
Gene expression library also requires a lot of primers. The traditional method of synthesizing degenerated primers often leads to increased redundancy of library, which not only brings the difficulty for subsequent screening, the probability of success is also greatly affected.
With advanced electrochemical technique, Creative Biogene can synthesize thousands of oligonucleotides in a semiconductor chip simultaneously. The oligonucleotides are cleaved from the surface of chip to create custom oligo pools.
Creative Biogene's custom oligo pool design service provides unparalleled advantages:
High throughput -- two platforms, up to 12,000 different oligos or 90,000 different oligos per pool.
High fidelity -- oligos with error rates as low as 0.5% (1 error in 200 bases).
The amount of each oligo is about 1 fmole.
Oligo length range from 10-200 bases.
Extremely competitive pricing -- as low as 0.04 cents per base.
For any more information, please don't hesitate to contact us.