Agomir/antagomir are members of a novel class of chemically engineered oligonucleotides. Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. The antisense strand has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 4 cholesterol groups at the 3' end, and full length nucleotide 2'-methoxy modification. Antagomir is single-strand miRNA inhibitor carrying the same modifications functioning by blocking miRNA regulation of target gene expression efficiently.
Creative Biogene provides agomir/ antagomir synthesis service that covers all human, mouse and rat miRNAs in the current miRBase. Our MicroRNA agomir/ antagomir synthesis service provides unparalleled advantages:
(Fig. a) It shows the interfering effect of antagomir miRNA is much better than other chemical modified miRNA.
(Fig. b) Antagomir can inhibit the target microRNA specifically. The effects of antagomir -122 were found to be speciﬁc because a control for antagomir -122 that harbored four mismatch mutations (mm- antagomir-122) had no effect on miR-122 levels. Furthermore, expression levels of let7 and miR-22 were unaffected treated with antagomir-122 and mm-antagomir-122, indicating that silencing was miRNA-speciﬁc.