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MicroRNA Sponge Service

email info@creative-biogene.com Tel: 1-631-626-9181 Fax: 1-631-614-7828

Creative Biogene provides world-class MicroRNA (miRNA) sponge service and we have been perfecting MicroRNA sponge service for many years. MicroRNA sponge is a RNA molecule with multiple tandem miRNA binding sequences that can sequester miRNAs from their endogenous target mRNAs and thus serve as a decoy , and a stably expressed miRNA sponge is particularly valuable for long-term loss-of-function studies both in vitro and in vivo.

Tandem miRNA binding sequences usually imperfectly match the target miRNA and are inserted in the 3′UTR of a reporter gene. The most common miRNA sponge design involves the insertion of a tandem multiplex artificial miRNA binding sequence into the 3′UTR of a Pol II-driven reporter gene, such as green fluorescence protein (GFP) or luciferase. In addition, there is another miRNA sponge system that utilizes the Pol III promoter to drive sponge expression, in which 5' and 3' stem-loop structures are added to stabilize the RNA products to substitute the reporter gene in the Pol II promoter system. With more and more noncoding RNAs were discovered, MicroRNA sponge has indispensable role in both scientific research and practical application.

Compared with antisense oligonucleotide inhibitor and genetic knockout, MicroRNA sponge possesses the following superiority

  • Due to encoded by plasmid, MicroRNA sponge can silence target MicroRNA stablely through eukaryotic expression vector and packaged lentivirus particle.
  • MicroRNA sponge is especially suitable for the functional research of a family of MicroRNAs because of the conserved seed sequences.
  • MicroRNA sponge is particularly valuable for long-term loss-of-function study both in vitro and in vivo.
  • Even completely unrelated miRNAs binding sequences can be constructed compatibly within one vector, so MicroRNA sponge could slience multiple miRNAs simultaneously.
 

Creative Biogene's MicroRNA sponge service is highly flexible for any specific project requirements to free your energy and time from routinely operating steps for more creative research. We can help you to speed up your work and we promise to delivery the service and product you need within shortest turnaround time, so that you can skip the technical hurdles, focus more on the biology your research, and expedite end-results.

Please contact us to learn more and discuss appropriate applications.

Our customer service representatives are available 24 hours a day, from Monday to Sunday.

  1. Ebert MS, Neilson JR and Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat. Methods 2007, 4: 721–726.
  2. Ebert MS and Sharp PA. MicroRNA sponges: Progress and possibilities. RNA 2010, 16: 2043-2050.

  3. Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J. Natural RNA circles function as efficient MicroRNA sponges. Nature 2013, 495, 384-388.
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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email:info@creative-biogene.com
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Tel: 44-207-048-3343

Email:info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-048-3343

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