Cat.No. : AD00275Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00275Z |
| Target Gene | VEGF |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | VEGF |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Stem cell therapy has gained momentum in the past few years, especially umbilical cord mesenchymal stem cells (MSCs) transplantation, which has great prospects in the treatment of non-healing ulcers and tissue regeneration due to their multi-directional differentiation potential. Human umbilical cord (hUC) is one of the most abundant tissues for MSCs. hUC-MSCs have many advantages: they have high self-replication and differentiation capacity both in vivo and in vitro; they have multi-directional differentiation potential. Here, hUC-MSCs cells overexpressed VEGF and were transplanted into a rat model of lower limb ischemia in type 2 diabetes. The study showed that VEGF overexpression increased hUC-MSCs cell proliferation activity and VEGF secretion. Transplantation of VEGF gene-transfected hUC-MSCs kept VEGF expression in rat skeletal muscle tissue at a high level for 4 weeks. Notably, vascular proliferation and blood perfusion in the limbs transplanted with VEGF-overexpressing hUC-MSCs were significantly improved compared with the control group. The expression of ERK, AKT, MMP2, and MMP9 in the VEGF-overexpressing hUC-MSCs transplantation group was significantly higher than that in the control group, while the expression of TIMP1 and TIMP2 did not change significantly. Therefore, VEGF-overexpressing hUC-MSCs transplantation can more effectively stimulate angiogenesis and increase blood perfusion than simple hUC-MSCs transplantation, and is expected to become a new option for improving lower limb vascular lesions in diabetic patients.
Here, to investigate the effect of VEGF overexpression on the proliferation activity of hUC-MSCs, cells were infected with 400 U Ad-VEGF recombinant adenovirus or control adenovirus for different time periods (24, 48, 72, 96 h). ELISA assay results showed that the VEGF content in the culture medium was higher than that in the control group throughout the 96 h period (Figure 1A). MTT assay results confirmed that VEGF overexpression increased cell proliferation activity in a time-dependent manner throughout the 96 h period (Figure 1B). FCS assay results showed that VEGF overexpression significantly increased the number of cells in the G0/G1 phase compared with the Ad control group (Figure 1C). These results confirmed that Ad-VEGF infection can increase the proliferation activity of hUC-MSCs.
Figure 1. Ad-VEGF infection increased the hUC-MSCs cell proliferation activity. (Li X, et al., 2015)
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