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LAM PCR Service

email info@creative-biogene.com Tel: 1-631-626-9181 Fax: 1-631-614-7828

Creative Biogene is a biotechnology company which has the expertise to provide you with customer LAM PCR service to analyze the viral vector insertion/integration site. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to assist you in all process that LAM PCR service needed. Besides, with the help of highly experienced staffs, Creative Biogene can provide the fastest turnaround time of any supplier in the industry.

LAM PCR service

LAM - PCR (Linear - amplification mediated PCR) is a technology which used for identifying and characterizing unknown flanking DNA adjacent to known DNA of any origin. More specifically, LAM-PCR has been developed to localize viral vector integration sites (IS) within the host genome [1, 2]. Genetic elements like retroviruses or transposons integrate their genome into the host genome in a (semi-) random manner [3-6]. In many cases it is decisive to know exactly the position where these vectors integrated. Customers now can use Creative Biogene’s most affordable, high-quality LAM PCR services to solve this problem.

Application:

  1. Analysis the viral vector integration sites in host genomes
  2. Localization of genetic elements and characterization of unknown flanking DNA
  3. Elucidating biological and clinical issues

Feature:

  1. Multiarm LAM-PCR service – can detect of low copy number, multiple unknown DNA insertion site
  2. High accuracy and sensitivity
  3. Competitive prices
  4. Fast turnaround time

Creative Biogene offers custom LAM PCR services for your scientific research as follows:

  1. Linear PCR
  2. Magnetic capture and double strand DNA (dsDNA) synthesis;
  3. Restriction digest and ligation of ds linker (LK)
  4. Denaturation of synthesized dsDNA and nested PCR
  5. Purification the PCR products and sequencing

Reference:
[1] Schmidt M, Hoffmann G, Wissler M, et al. Detection and direct genomic sequencing of multiple rare unknown flanking DNA in highly complex samples. Human gene therapy, 2001, 12(7): 743-749.
[2] Schmidt M, Schwarzwaelder K, Bartholomae C, et al. High-resolution insertion-site analysis by linear amplification–mediated PCR (LAM-PCR). Nature methods, 2007, 4(12): 1051-1057.
[3] Harkey MA, Kaul R, Jacobs MA, et al. Multiarm High-Throughput Integration Site Detection: Limitations of LAM-PCR Technology and Optimization for Clonal Analysis. Stem Cells Dev. 2007, 16(3):381-92.
[4] Schr?der ARW, Shinn P, Chen H, et al. HIV-1 integration in the human genome favors active genes and local hotspots. Cell, 2002, 110(4): 521-529.
[5] Wu X, Li Y, Crise B, et al. Transcription start regions in the human genome are favored targets for MLV integration. Science, 2003, 300(5626): 1749-1751.
[6] Vigdal T J, Kaufman C D, Izsvák Z, et al. Common physical properties of DNA affecting target site selection of sleeping beauty and other Tc1/mariner transposable elements. Journal of molecular biology, 2002, 323(3): 441-452.

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email:info@creative-biogene.com
Europe
Tel: 44-207-048-3343

Email:info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-048-3343

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