Pages
Products

EPCAM


Official Full Name
epithelial cell adhesion molecule
Organism
Homo sapiens
Gene ID
4072
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Synonyms
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; BerEp4; EGP314; HNPCC8; LYNCH8; MOC-31; Ber-Ep4; TACSTD1

Cat.No. Product Name Price
SHH286297 shRNA set against Mouse EPCAM (NM_008532.2) Inquiry
SHH286301 shRNA set against Rat EPCAM (NM_138541.1) Inquiry
SHW000878 shRNA set against Chicken EPCAM (NM_001012564) Inquiry
SHH342236 shRNA set against Rat Mk1 (NM_134399.2) Inquiry
SHL053748 shRNA set against Rat Tacstd1(NM_138541.1) Inquiry
SHL053766 shRNA set against Mouse Tacstd1(NM_008532.2) Inquiry
SHW008255 shRNA set against Danio rerio EPCAM (NM_001017593) Inquiry
SHW013864 shRNA set against Danio rerio SLC25A38B (NM_001202458) Inquiry
Cat.No. Product Name Price
CDCL184059 Rat EPCAM ORF clone(NM_138541.1) Inquiry
CDCR245639 Mouse Epcam ORF Clone(NM_008532.2) Inquiry
CDCS418503 Human EPCAM ORF Clone (BC014785) Inquiry
CDFR014192 Rat Mk1 cDNA Clone(NM_134399.2) Inquiry
CDFR014244 Rat Epcam cDNA Clone(NM_138541.1) Inquiry
MiUTR1M-11491 EPCAM miRNA 3'UTR clone Inquiry
MiUTR1R-03927 MK1 miRNA 3'UTR clone Inquiry
MiUTR1R-07877 EPCAM miRNA 3'UTR clone Inquiry
MiUTR3H-02150 EPCAM miRNA 3'UTR clone Inquiry
CDCB162353 Chicken EPCAM ORF Clone (NM_001012564) Inquiry
CDCB169730 Danio rerio EPCAM ORF Clone (NM_001017593) Inquiry
CDCB175339 Danio rerio SLC25A38B ORF Clone (NM_001202458) Inquiry
CDCB190149 Rabbit EPCAM ORF clone (XM_002709724.2) Inquiry
CDCL184058 Human EpCAM ORF clone(NM_002354.2) Inquiry
CDCR381217 Rat Mk1 ORF Clone(NM_134399.2) Inquiry

Detailed Information

EpCAM, also known as TACSTD1 (tumor-associated calcium signal transducer protein 1), is a single-transmembrane type I glycoprotein according to the leukocyte differentiation antigen as CD326. The EpCAM molecular structure consists of an extracellular domain, a single transmembrane domain and an intracellular domain, which encodes a tumor-associated antigen. EpCAM is expressed in normal epithelial cells and epithelial-derived malignant cells.

The Biological Function of EpCAM

EpCAM was first recognized in mouse fibroblasts. Its biological functions include cell adhesion, proliferation, maintenance of undifferentiated state, and regulation of differentiation. Studies have shown that EpCAM shows carcinogenic properties in certain in vitro cell models. EpCAM plays an important role in tumor development and invasion, and can increase the migration and proliferation of tumor cells. Overexpression of EpCAM may result in activation of the canonical signaling pathway of the Wnt-β-catenin signaling pathway, which activates the expression of the proto-oncogenes e-myc and cyclin A/E via the Wnt cascade to induce cell proliferation. EpCAM impairs E-cadherin-mediated adhesion and may result in an increase in non-membrane-bound β-catenin.

Figure 1. EpCAM signaling in cross-talk with E-Cadherin. (Schnell, et al. 2013)

AATF and Tumor

EpCAM is highly expressed in gastric cancer tissues and cell lines, and down-regulation of EpCAM by RNA interference leads to a decrease in cell proliferation and cell cycle arrest. Related studies have confirmed the high expression of EpCAM in gastric cancer and its prognosis. EpCAM is negative in normal esophageal epithelium, but in primary esophageal squamous cell carcinoma, approximately 80% have abnormal expression in varying degrees and are associated with prognosis. EpCAM was first discovered in colon cancer in 1979. A study of tissue microarrays by immunohistochemistry showed high levels of EpCAM expression in colon cancer.

It has been reported that EpCAM binds tightly to the protein claudin-7 to regulate the tight junctions between ovarian cancer cells, which promotes cell proliferation, apoptosis resistance and tumorigenicity. EpCAM and claudin-7 form the EpCAM-claudin-7 complex, which can increase the migration, proliferation and anti-cisplatin treatment of HEK293 cells. In vivo, the EpCAM-claudin-7 complex promotes the development of pancreatic tumors in rats and the dissemination of tumor cells. This suggests that EpCAM has a tumor promoting effect. Studies have shown that EpCAM directly mediates cell-cell adhesion, inhibits substrate and EGF-induced migration of human ovarian cancer cells, overexpresses EpCAM to reduce cell migration, and knocks out EpCAM to increase cell migration.

The high expression of EpCAM is important in the treatment of epithelial tumors. EpCAM antibodies or vaccines have been widely used in various clinical tumor immunotherapy trials, including monoclonal antibodies and antibody fragments. In 2009, the first anti-EpCAM antibody approved by the European Commission was named catumaxomab, which is effective in the treatment of malignant ascites in patients with EpCAM-positive tumors. The effect of rituximab is based on its binding to the surface EpCAM of tumor cells, CD3 antigen binding on the surface of T cells, and FC receptor binding on the surface of helper cells (such as natural killer cells, dendritic cells, macrophages). Through T cell-mediated cell lysis, antibody-dependent cell-mediated cytotoxicity, rituximab phagocytose and kill tumor cells.

References:

  1. Schnell, U., Cirulli, V., & Giepmans, B. N. (2013). Epcam: structure and function in health and disease. BBA - Biomembranes, 1828(8), 1989-2001.
  2. Fan, Q., Cheng, J. C., Qiu, X., Chang, H. M., & Leung, P. C. (2015). Epcam is up-regulated by egf via erk1/2 signaling and suppresses human epithelial ovarian cancer cell migration. Biochem Biophys Res Commun,457(3), 256-261.
Quick Inquiry