Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-DC011538 | Panoply™ Human PDK1 Knockdown Stable Cell Line | Inquiry |
| CSC-DC011550 | Panoply™ Human PDPK1 Knockdown Stable Cell Line | Inquiry |
| CSC-SC011538 | Panoply™ Human PDK1 Over-expressing Stable Cell Line | Inquiry |
| CSC-SC011550 | Panoply™ Human PDPK1 Over-expressing Stable Cell Line | Inquiry |
| CSC-RT0063 | Human PDPK1 Knockout Cell Line-HCT116 | Inquiry |
| CSC-RT0835 | Human PDK1 Knockout Cell Line-HeLa | Inquiry |
| CLOE-0628 | Human PDK1 Insect Cell Lysate | Inquiry |
| CLKO-0338 | PDK1 KO Cell Lysate-HeLa | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| AD12037Z | Human PDK1 adenoviral particles | Inquiry |
| AD12049Z | Human PDPK1 adenoviral particles | Inquiry |
| LV21400L | human PDK1 (NM_002610) lentivirus particles | Inquiry |
| LV21423L | human PDPK1 (NM_031268) lentivirus particles | Inquiry |
| LV21424L | human PDPK1 (NM_002613) lentivirus particles | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| SHH373012 | shRNA set against Rat PDPK1 (NM_031081.1) | Inquiry |
| SHR102300 | shRNA set against Mouse Pdpk1(NM_001080773.1) | Inquiry |
| SHR103062 | shRNA set against Rat Pdpk1(NM_031081.1) | Inquiry |
| SHW000846 | shRNA set against Chicken PDPK1 (NM_001012529) | Inquiry |
| SHH365820 | shRNA set against Rat Olr754 (NM_001000368.1) | Inquiry |
| SHH372864 | shRNA set against Human PDK1 (NM_002610.3) | Inquiry |
| SHH372868 | shRNA set against Mouse PDK1 (NM_172665.4) | Inquiry |
| SHH372872 | shRNA set against Rat PDK1 (NM_053826.2) | Inquiry |
| SHH373008 | shRNA set against Mouse PDPK1 (NM_011062.4) | Inquiry |
| SHR067656 | shRNA set against Rat Olr77_predicted(NM_001001287.1) | Inquiry |
| SHR102228 | shRNA set against Rat Pdk1(NM_053826.2) | Inquiry |
| SHR102264 | shRNA set against Human PDK1(NM_002610.3) | Inquiry |
| SHR102354 | shRNA set against Mouse Pdpk1(NM_011062.4) | Inquiry |
| SHW001947 | shRNA set against Chicken PDK1 (NM_001031352) | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| CDFH013992 | Human PDK1 cDNA Clone(NM_002610.3) | Inquiry |
| MiUTR1R-05733 | PDK1 miRNA 3'UTR clone | Inquiry |
| MiUTR1R-05377 | OLR754 miRNA 3'UTR clone | Inquiry |
| MiUTR1M-09077 | PDPK1 miRNA 3'UTR clone | Inquiry |
| MiUTR1H-07755 | PDPK1 miRNA 3'UTR clone | Inquiry |
| MiUTR1H-07754 | PDPK1 miRNA 3'UTR clone | Inquiry |
| MiUTR1H-07753 | PDPK1 miRNA 3'UTR clone | Inquiry |
| MiUTR1H-07744 | PDK1 miRNA 3'UTR clone | Inquiry |
| CDFR013563 | Rat Pdk1 cDNA Clone(NM_053826.2) | Inquiry |
| CDFR012552 | Rat Pdpk1 cDNA Clone(NM_031081.1) | Inquiry |
| CDFR000360 | Rat Olr754 cDNA Clone(NM_001000368.1) | Inquiry |
| CDFH013993 | Human PDK1 cDNA Clone(NM_002610.3) | Inquiry |
| MiUTR1R-05742 | PDPK1 miRNA 3'UTR clone | Inquiry |
| SKO0199 | PDK1 Validated sgRNA vector | Inquiry |
| CDCL185686 | Mouse PDPK1 ORF clone(NM_011062.4) | Inquiry |
| CDCB162321 | Chicken PDPK1 ORF Clone (NM_001012529) | Inquiry |
| CDFG018719 | Mouse Pdpk1 cDNA Clone(NM_001080773.1) | Inquiry |
| CDCS410862 | Human PDK1 ORF Clone (BC039158) | Inquiry |
| CDCR380595 | Rat Pdk1 ORF Clone(NM_053826.2) | Inquiry |
| CDCR379574 | Rat Pdpk1 ORF Clone(NM_031081.1) | Inquiry |
| CDCR367437 | Rat Olr754 ORF Clone(NM_001000368.1) | Inquiry |
| CDCR283606 | Human PDK1 ORF Clone(NM_002610.3) | Inquiry |
| CDCL147783 | Mouse Pdpk1 ORF clone (NM_001080773.1) | Inquiry |
| CDCL147663 | Mouse Pdk1 ORF clone (NM_172665.4) | Inquiry |
| CDCB187082 | Rabbit PDK1 ORF clone (XM_008258748.1) | Inquiry |
| CDCB163422 | Chicken PDK1 ORF Clone (NM_001031352) | Inquiry |
| CDCB160100 | Human PDK1 ORF clone (BC039158) | Inquiry |
Introduction
The serine-threonine kinase PDK1 (or PDPK1) is a key element of signaling transduction activated by extracellular ligands, which recognized as a key regulator of cell migration and chemotaxis by controlling numerous elements of signaling transduction and cytoskeletal dynamics. PDK1 has been proved to exist in different cell types and organisms including endothelial cells, mammary epithelial cells, smooth muscle cells, T lymphocytes and neutrophils. Furthermore, PDK1 gene ablation or silencing is associated with tumor progression in different cellular and experimental models.
PDK1 and the Cell Migration
PDK1 (an ancient and conserved protein kinase) is found throughout the entire eukaryotic domain, whose importance in vertebrates is reflected in its relevant physiological roles in embryonic development and adulthood. PDK1 is a member of the AGC kinase family (cAMP-dependent, cGMP-dependent and protein kinase C), which includes 60 serine-threonine kinases with a common phylogenetic origin. PDK1 is responsible for the phosphorylation and activation of several other AGC kinases, such as p70S6K, SGK, p90RSK and atypical PKC isoforms, and is initially characterized for the phosphorylation of Akt on threonine 308 in the activation loop, which is essential for its activation. PDK1 is mainly localized in the cytoplasm and able to translocate into the nucleus with a mechanism that involves the inhibition of its nuclear export sequence. Previous studies have shown that PDK1 can move to the plasma membrane, which is essential for its ability to regulate cell migration.
PDK1 and the Signaling Pathways of Cell Migration
It is reported that the binding of PDK1 to plasma membrane phospholipid PtdIns (3, 4, 5) P3 produced by means of PI3K activity is one prominent explanation of the relevant role of PDK1 in regulating cell migration. PDK1 and Akt share the ability to bind PtdIns (3,4,5) P3 through their PH domains. When they colocalize at the plasma membrane, PDK1 phosphorylates Akt activation loop on threonine 308. PAK1 can bind the small GTPases cdc42 and RAC1, which event could potentially colocalize PDK1 and PAK1 at the plasma membrane at the leading edge. Moreover, PAK1 was shown involved in the regulation of cell migration through its substrates LIMK, p41-ARC, filamin A.
PDK1 Control Different Hallmarks of Tumor Invasion
Tumors may be defined as a wide group of somatic diseases accompanied by the uncontrolled proliferation of cells. The ability to invade surrounding tissues is considered a distinctive feature of malign ones. It has been reported that PDK1 plays a crucial role in different processes involved in tumor invasion and dissemination. PI3K/PDK1/Akt signaling axis is also required to form functional invadopodia with proteolytic activity as shown by in vitro gelatin degradation, but PDK1 and Akt inhibition provides an effective tool to block invadopodia formation which is sustained by the active and oncogenic forms of p110α (E545K and H1047R). PDK1 is involved in the regulation of collective invasion in MCF10DCIS model of breast tumor by MRCKα signaling pathway, whose overexpression leads to the formation of multicellular invasive structures from MCF10DCIS spheroids in basement membrane gel.
Figure 1. PDK1 localizes at the leading edge of migrating cells by binding to PtdIns (3,4,5) P3 produced by PI3K. (http://dx.doi.org/10.1016/j.bbcan.2015.07.003)
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