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PLEC


Official Full Name
plectin
Organism
Homo sapiens
Gene ID
5339
Background
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011]
Synonyms
HD1; PCN; EBS1; EBSO; PLTN; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD; EBSND; EBSOG; EBSPA; PLEC1; LGMD2Q; PLEC1b; LGMDR17

Cat.No. Product Name Price
SHH377416 shRNA set against Human PLEC (NM_000445.3) Inquiry
SHH377420 shRNA set against Mouse PLEC (NM_011117.2) Inquiry
SHH377424 shRNA set against Rat PLEC (NM_022401.2) Inquiry
SHR121314 shRNA set against Human PLEC1(NM_201383.1) Inquiry
SHR121368 shRNA set against Human PLEC1(NM_201380.2) Inquiry
Cat.No. Product Name Price
CDCR326930 Human PLEC ORF Clone(NM_201378.2) Inquiry
MiUTR3H-04028 PLEC miRNA 3'UTR clone Inquiry
MiUTR1H-07993 PLEC miRNA 3'UTR clone Inquiry
MiUTR1H-07992 PLEC miRNA 3'UTR clone Inquiry
MiUTR1H-07991 PLEC miRNA 3'UTR clone Inquiry
MiUTR1H-07990 PLEC miRNA 3'UTR clone Inquiry
MiUTR1H-07989 PLEC miRNA 3'UTR clone Inquiry
CDFR011849 Rat Plec cDNA Clone(NM_022401.2) Inquiry
CDFG013195 Human PLEC cDNA Clone(NM_201380.2) Inquiry
CDFG013194 Human PLEC cDNA Clone(NM_201384.1) Inquiry
MiUTR3H-04029 PLEC miRNA 3'UTR clone Inquiry
CDFG013193 Human PLEC cDNA Clone(NM_201383.1) Inquiry
CDFG013190 Human PLEC cDNA Clone(NM_201379.1) Inquiry
CDFG013191 Human PLEC cDNA Clone(NM_201381.1) Inquiry
MiUTR3H-04033 PLEC miRNA 3'UTR clone Inquiry
CDCR379008 Rat Plec ORF Clone(NM_022401.2) Inquiry
CDCR336416 Human PLEC ORF Clone(NM_000445.3) Inquiry
CDCR326942 Human PLEC ORF Clone(NM_201384.1) Inquiry
CDCR326940 Human PLEC ORF Clone(NM_201383.1) Inquiry
CDCR326938 Human PLEC ORF Clone(NM_201382.2) Inquiry
CDCR326937 Human PLEC ORF Clone(NM_201381.1) Inquiry
CDCR326934 Human PLEC ORF Clone(NM_201380.2) Inquiry
CDCR326932 Human PLEC ORF Clone(NM_201379.1) Inquiry
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