BRIP1

Official Full Name

BRCA1 interacting DNA helicase 1

Organism

Homo sapiens

Gene ID

83990

Background

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]

Synonyms

OF; BACH1; FANCJ

Cat.No.	Product Name	Price
CSC-DC001538	Panoply™ Human BRIP1 Knockdown Stable Cell Line	Inquiry
CSC-DC009744	Panoply™ Human MRPL36 Knockdown Stable Cell Line	Inquiry
CSC-SC001538	Panoply™ Human BRIP1 Over-expressing Stable Cell Line	Inquiry
CSC-SC009744	Panoply™ Human MRPL36 Over-expressing Stable Cell Line	Inquiry

Cat.No.	Product Name	Price
AD01911Z	Human BRIP1 adenoviral particles	Inquiry
AD10171Z	Human MRPL36 adenoviral particles	Inquiry
LV06346L	human BRIP1 (NM_032043) lentivirus particles	Inquiry
LV18565L	human MRPL36 (NM_032479) lentivirus particles	Inquiry

Cat.No.	Product Name	Price
SHH200279	shRNA set against Mouse Mrpl36(NM_053163.1)	Inquiry
SHH247742	shRNA set against Human BRIP1 (NM_032043.2)	Inquiry
SHH247746	shRNA set against Mouse BRIP1 (NM_178309.2)	Inquiry
SHH332471	shRNA set against Rat LOC500702 (NM_001047960.1)	Inquiry
SHH344788	shRNA set against Human MRPL36 (NM_032479.3)	Inquiry
SHH344792	shRNA set against Mouse MRPL36 (NM_053163.1)	Inquiry
SHH344796	shRNA set against Rat MRPL36 (NM_001108879.1)	Inquiry
SHW002202	shRNA set against Chicken BRIP1 (NM_001033058)	Inquiry
SHW011638	shRNA set against Danio rerio MRPL36 (NM_001099257)	Inquiry
SHW012022	shRNA set against Danio rerio BRIP1 (NM_001110296)	Inquiry

Cat.No.	Product Name	Price
MiUTR3H-12145	MRPL36 miRNA 3'UTR clone	Inquiry
MiUTR1R-03448	LOC500702 miRNA 3'UTR clone	Inquiry
MiUTR1M-07278	MRPL36 miRNA 3'UTR clone	Inquiry
CDFR008447	Rat Mrpl36 cDNA Clone(NM_001108879.1)	Inquiry
CDFR004941	Rat LOC500702 cDNA Clone(NM_001047960.1)	Inquiry
MiUTR3H-08017	BRIP1 miRNA 3'UTR clone	Inquiry
CDCS419059	Human MRPL36 ORF Clone (BC020642)	Inquiry
CDCS416152	Human BRIP1 ORF Clone (BC101472)	Inquiry
CDCR375502	Rat Mrpl36 ORF Clone(NM_001108879.1)	Inquiry
CDCR274752	Mouse Brip1 ORF Clone(NM_178309.2)	Inquiry
CDCL134093	Mouse Mrpl36 ORF clone (NM_053163.1)	Inquiry
CDCB190440	Rabbit MRPL36 ORF clone (XM_008252888.1)	Inquiry
CDCB182307	Rabbit BRIP1 ORF clone (XM_008271191.1)	Inquiry
CDCB173497	Danio rerio BRIP1 ORF Clone (NM_001110296)	Inquiry
CDCB173113	Danio rerio MRPL36 ORF Clone (NM_001099257)	Inquiry
CDCR372016	Rat LOC500702 ORF Clone(NM_001047960.1)	Inquiry
CDCB163677	Chicken BRIP1 ORF Clone (NM_001033058)	Inquiry

Detailed Information

BRCA1 interacting protein 1 (BRIP1), which cooperates with many DNA metabolic proteins involved in DNA damage detection and repair, and plays an important role in cell cycle checkpoint control. BRIP1 is a DNA-dependent ATPase and a 5'-3' DNA helicase, which achieves its cancer suppression function and DNA double-strand break repair through direct interaction with a highly conserved C-terminal BRCT (BRCA1 C-terminal domain) protein domain repeats of the tumor suppressor BRCA1. The N-terminal 888-residue structure of BRIP1 shows strong homology to the catalytic and nucleotide binding domains of the DEAH helicase family members. Abnormal BRIP1 function contributes to tumor induction. In fact, BRCA1 repeats mutations within BRCT1 disrupting its interaction with BRIP1 and leading to DNA repair defects leading to multiple forms of cancer.

BRIP1 and cervical cancer

Cervical cancer is the second most common cancer among women worldwide and is an important cause of morbidity and mortality rate. Liu et al. constructed a BRIP1 recombinant plasmid that was overexpressed in the cervical cancer cell line (HeLa) and found that ectopic expression of BRIP1 significantly enhanced the antitumor activity of cisplatin. In addition, BRIP1 promoted cisplatin-mediated apoptosis and inhibited tumor angiogenesis. Synergistic inhibition of BRIP1 has been reported to be partially due to attenuated Rac1 GTPase activation, and Rac1 GTPase reactivation could reverse BRIP1-induced sensitization. In conclusion, up-regulation of BRIP1 can enhance the chemosensitivity of HeLa cells to cisplatin by inhibiting the activation of Rac1 GTPase, providing new insights into the important role of BRIP1 in cervical cancer chemotherapy.

BRIP1 and HCC

Hepatocellular carcinoma (HCC) is the most common primary malignancy in the liver. The BRIP1 locus is closely associated with HCC risk in patients with HBV- and/or HCV-induced liver disease, even after adjusting for age, sex, body mass index, alcohol consumption, aminotransferase levels, disease duration, viral cirrhosis etiology, and potential population admixture. The current research results show investigating in experimental models the BRIP1 phosphorylation pathway and its interaction with BLM as a possible mechanisms of liver carcinogenesis. Previous preclinical studies have shown that PARP inhibition is a potentially promising HCC treatment strategy. Recent studies have revealed that BRIP1 genotyping should be used to predict the efficacy of forthcoming trials of HCC treatments that will target this pathway.

BRIP1 and SCCHN

The incidence of global head and neck squamous cell carcinoma (SCCHN) has increased significantly in the last 10 years, especially in women. Recently, the study found that single nucleotide polymorphisms (SNPs) rs7213430 in BRIP1 was significantly associated with SCCHN risk. In addition, functional analysis revealed that SNP rs7213430 is in the miR-101 seed binding region, and variant G alleles could result in significantly lower luciferase activity and BRIP1 mRNA expression, compared to the A allele with the presence of miR-101. The current results indicate that SNP rs7213430 in the 3'-UTR of BRIP1 might cause SCCHN susceptibility by affecting the binding activity of miR-101 and lead to a decrease in BRIP1 expression.

In conclusion, BRIP1 is required for BRCA-associated DNA damage repair function and may be involved in tumorigenesis and invasiveness of various cancers. Therefore, further study of the function of BRIP1 and its mechanism of action in cancer will provide new insights into the treatment of related diseases.

References:

Abderrahim O, et al. BRIP1 coding variants are associated with a high risk of hepatocellular carcinoma occurrence in patients with HCV- or HBV-related liver disease. Oncotarget, 2017, 8(38): 62842-62857
Wei Z, et al. BRIP1 inhibits the tumorigenic properties of cervical cancer by regulating RhoA GTPase activity. Oncology Letters, 2016, 11: 551-558
Liu HL, et al. A variant at a potentially functional microRNA-binding site in BRIP1 was associated with risk of squamous cell carcinoma of the head and neck. Tumour Biol, 2016, 37(6): 8057–8066
Ishita Gupta, et al. BRIP1 overexpression is correlated with clinical features and survival outcome of luminal breast cancer subtypes. Endocrine Connections, 2018, 7: 65–77

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