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P4HA3


Official Full Name
prolyl 4-hydroxylase subunit alpha 3
Organism
Homo sapiens
Gene ID
283208
Background
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Synonyms
THAP12; DAP4; THAP0; PRKRIR; P52rIPK

Cat.No. Product Name Price
SHH369664 shRNA set against Human P4HA3 (NM_182904.3) Inquiry
SHH369668 shRNA set against Mouse P4HA3 (NM_177161.4) Inquiry
SHH369672 shRNA set against Rat P4HA3 (NM_198775.1) Inquiry
SHR076854 shRNA set against Human P4HA3(NM_182904.3) Inquiry
SHR085884 shRNA set against Rat P4ha3(NM_198775.1) Inquiry
Cat.No. Product Name Price
CDFG012252 Human P4HA3 cDNA Clone(NM_182904.3) Inquiry
CDFL009827 Mouse P4ha3 cDNA Clone(NM_177161.4) Inquiry
CDFR015179 Rat P4ha3 cDNA Clone(NM_198775.1) Inquiry
MiUTR1H-07450 P4HA3 miRNA 3'UTR clone Inquiry
MiUTR1R-05586 P4HA3 miRNA 3'UTR clone Inquiry
CDCB184269 Rabbit P4HA3 ORF clone (XM_008264084.1) Inquiry
CDCR273595 Mouse P4ha3 ORF Clone(NM_177161.4) Inquiry
CDCR324856 Human P4HA3 ORF Clone(NM_182904.3) Inquiry
CDCR382230 Rat P4ha3 ORF Clone(NM_198775.1) Inquiry
CDCS417726 Human P4HA3 ORF Clone (BC117333) Inquiry
CDCS417727 Human P4HA3 ORF Clone (BC126170) Inquiry

Detailed Information

P4HA3 functions as cancer promoter in head and neck squamous cell carcinoma by epithelial-mesenchymal transition regulation

As one of the most common malignancies worldwide, head and neck squamous cell carcinoma is characterized by local invasion and metastasis. Continuous improvement in treatment regimens for head and neck squamous cell carcinoma have improved over the past 30 years, and it is synchronized with the modifying prognosis of patients. Distant metastasis of this disease can lower down the nearly 50% survival rate in the five-year into 20%. And it may reflect the inadequacy of the current regimens. In view of the key role, biomarkers played in clinical application for assisting in diagnosis and prognosis, or aiding suitable selection of the treatment. Bioinformatics analysis demonstrated that there is an up-regulation of P4HA3 in head and neck squamous cell carcinoma tissues, more than this, P4HA3 can perform an independent prognostic value for head and neck squamous cell carcinoma patients.

P4HA3 activated by Slug epigenetically in Gastric cancer

As the alpha subunit of enzymatic active site of prolyl 4-hydroxylase, prolyl 4-hydroxylase alpha subunit is a critical enzyme for the stability maintain of newly synthesized collagens. Expression profile of P4HA3 RNA was found to be significantly elevated in gastric cancer than in normal stomach tissues in the database of Cancer Genome Atlas-Stomach Cancer. However, Prolyl 4-hydroxylase alpha subunit cannot be detected in normal stomach tissues, but has little staining in 7 of 12 gastric cancer tissues by the immunohistochemistry staining method. SNAI2, an encoding slug, has a high expression with P4HA3 at Pearson r value of 0.70 in the database of Cancer Genome Atlas-stomach Cancer. Efficiently binding with P4HA3 promoter and increasing its transcription can be achieved in the in vitro cell assay. In cancer Genome Alas-case database or date mining from the Kaplan-Meier plotter, high P4HA3 expression had relatedness with the unfavorable overall survival rate and first progression-free survival. Therefore, deregulation of the slug epigenetically activated P4HA3 has associations with poor survival and metastasis enhancement of the gastric cancer.  

Contribution of P4HA3 in the procollagen molecule formation as drivers of tumor metastasisFigure 1. Contribution of P4HA3 in the procollagen molecule formation as drivers of tumor metastasis (Daniele M. Gilkes, et al. 2014)

References:

  1. Wang T, Wang Y X, Dong Y Q, et al. Prolyl 4-hydroxylase subunit alpha 3 presents a cancer promotive function in head and neck squamous cell carcinoma via regulating epithelial-mesenchymal transition. Archives of oral biology, 2020, 113: 104711.
  2. Song H, Liu L, Song Z, et al. P4HA3 is epigenetically activated by slug in gastric cancer and its deregulation is associated with enhanced metastasis and poor survival. Technology in cancer research & treatment, 2018, 17: 1533033818796485.
  3. Gilkes D M, Semenza G L, Wirtz D. Hypoxia and the extracellular matrix: drivers of tumour metastasis. Nature Reviews Cancer, 2014, 14(6): 430-439.
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