P4HA3

Official Full Name

prolyl 4-hydroxylase subunit alpha 3

Organism

Homo sapiens

GeneID

283208

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Cat.No.	Product Name	Price

Cat.No.	Product Name	Price

Cat.No.	Product Name	Price

Cat.No.	Product Name	Price

Detailed Information

P4HA3 functions as cancer promoter in head and neck squamous cell carcinoma by epithelial-mesenchymal transition regulation

As one of the most common malignancies worldwide, head and neck squamous cell carcinoma is characterized by local invasion and metastasis. Continuous improvement in treatment regimens for head and neck squamous cell carcinoma have improved over the past 30 years, and it is synchronized with the modifying prognosis of patients. Distant metastasis of this disease can lower down the nearly 50% survival rate in the five-year into 20%. And it may reflect the inadequacy of the current regimens. In view of the key role, biomarkers played in clinical application for assisting in diagnosis and prognosis, or aiding suitable selection of the treatment. Bioinformatics analysis demonstrated that there is an up-regulation of P4HA3 in head and neck squamous cell carcinoma tissues, more than this, P4HA3 can perform an independent prognostic value for head and neck squamous cell carcinoma patients.

P4HA3 activated by Slug epigenetically in Gastric cancer

As the alpha subunit of enzymatic active site of prolyl 4-hydroxylase, prolyl 4-hydroxylase alpha subunit is a critical enzyme for the stability maintain of newly synthesized collagens. Expression profile of P4HA3 RNA was found to be significantly elevated in gastric cancer than in normal stomach tissues in the database of Cancer Genome Atlas-Stomach Cancer. However, Prolyl 4-hydroxylase alpha subunit cannot be detected in normal stomach tissues, but has little staining in 7 of 12 gastric cancer tissues by the immunohistochemistry staining method. SNAI2, an encoding slug, has a high expression with P4HA3 at Pearson r value of 0.70 in the database of Cancer Genome Atlas-stomach Cancer. Efficiently binding with P4HA3 promoter and increasing its transcription can be achieved in the in vitro cell assay. In cancer Genome Alas-case database or date mining from the Kaplan-Meier plotter, high P4HA3 expression had relatedness with the unfavorable overall survival rate and first progression-free survival. Therefore, deregulation of the slug epigenetically activated P4HA3 has associations with poor survival and metastasis enhancement of the gastric cancer.

Figure 1. Contribution of P4HA3 in the procollagen molecule formation as drivers of tumor metastasis (Daniele M. Gilkes, et al. 2014)

References:

Wang T, Wang Y X, Dong Y Q, et al. Prolyl 4-hydroxylase subunit alpha 3 presents a cancer promotive function in head and neck squamous cell carcinoma via regulating epithelial-mesenchymal transition. Archives of oral biology, 2020, 113: 104711.
Song H, Liu L, Song Z, et al. P4HA3 is epigenetically activated by slug in gastric cancer and its deregulation is associated with enhanced metastasis and poor survival. Technology in cancer research & treatment, 2018, 17: 1533033818796485.
Gilkes D M, Semenza G L, Wirtz D. Hypoxia and the extracellular matrix: drivers of tumour metastasis. Nature Reviews Cancer, 2014, 14(6): 430-439.

Quick Inquiry

Interested in learning more?

Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.

Request a quote today!

Inquiry