Membrane-anchored forms of mesothelin may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation. Mesothelin interacts with MUC16. The protein is expressed in lung and at low levels in the heart, placenta, kidney, and mesothelial cells. Mestothelin is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Both MPF and the cleaved form of mesothelin are N-glycosylated. It is proteolytically cleaved by a furin-like convertase to generate megakaryocyte- potentiating factor (MPF), and the cleaved form of mesothelin.