Official Full Name
minichromosome maintenance complex component 3 associated protein
GANP (germinal center-associated nuclear protein) is up-regulated selectively in germinal centers and carries an MCM-binding domain in the carboxyl-terminal side. It was found that a region (from 414 to 550 aa) in GANP molecule that is slightly similar to the known DNA-primase component p49. The recombinant GANP fragment covering this region synthesizes RNA primers for extension by DNA polymerase I with single-stranded DNA templates in vitro. GANP DNA-primase activity is controlled by phosphorylation at Ser(502) that is induced by CD40-mediated signaling in vitro and in the germinal center B cells stimulated with antigen in vivo. Overexpression of ganp cDNA in Daudi B cells caused the increased DNA synthesis more than the levels of the mock-transfectants. These evidences suggested that the novel DNA-primase GANP is involved in regulation of cell proliferation of antigen-driven B cells in germinal centers.
MCM3AP; minichromosome maintenance complex component 3 associated protein; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein , minichromosome maintenance deficient (S. cerevisiae) 3 associated protein; 80 kDa MCM3-associated protein; GANP; KIAA0572; Map80; MCM3 import protein; germinal center-associated nuclear protein; minichromosome maintenance deficient 3-associated protein; MCM3 minichromosome maintenance deficient 3 associated protein; FLJ44336; FLJ45306