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HepG2 Cell Line


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HepG2 Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionHuman hepatocellular carcinoma cells were isolated from a liver biopsy of a 15 years old male caucasian donor. And the donor had a well differentiated hepatocellular carcinoma. These cells were NOT isolated at CET but are sold to you under an unrestricted use license. HepG2 cells have been used to study a variety of phenomena including liver metabolism, tumor formation and cancer metastasis. These cells secrete many major plasma proteins including albumin, α2-macroglobulin, α1-antitrypsin, and plasminogen and transferrin.
Tissue of OriginLiver
Age15 years adolescent
GenderMale
EthnicityCaucasian
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
Biosafety Level1
Appropriate safety procedures should be used with this material. Laboratory safety is discussed in the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes for Health.
ApplicationsThese cells are suitable as a transfection host.
Shipped inDry ice
Storage Temperature−196°C
Characteristics
KaryotypeModal number = 55; range = 50 to 60; has a rearranged chromosome 1
ImagesHepG2 Cell Line
CommentsHepG2 cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.
The cells demonstrate decreased expression of apoAI mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress).
There is no evidence of a Hepatitis B virus genome in the cell line.
Genes Expressedα-fetoprotein; albumin; α1-antitrypsin; transferrin; α1-antichymotrypsin; α2-macroglobulin; haptoglobin; ceruloplasmin; plasminogen; C3 activator; fibrinogen; α1-acid glycoprotein; α2-HS glycoprotein; β-lipoprotein; retinol binding protein
TumorigenicNo
EffectsYes, in semisolid medium
No, in immunosuppressed mice
Culture Conditions and Handling
Culture MediumIn order to make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes are given for a 75 cm2 flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard the culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor
  3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (Generally within 5 to 15 minutes).
  4. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels
  6. Incubate cultures at 37°C.
Subcultivation RatioThe ratio of 1:4 to 1:6 is recommended.
Fluid Renewal2  times per week
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereAir, 95%; carbon dioxide (CO2), 5%

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