Baculovirus Services

Brief Introduction

Fig. 1. Life cycle of baculoviruses.[4]

Baculovirus are large double-stranded enveloped viruses consisting of circular DNA genomes. Since 1983, when the baculovirus expression system (BES) technology was first used to express human beta interferon in insect cells, the BES has become a versatile and robust eukaryotic expression system for foreign protein expression. Baculoviruses have several advantages over other expression vector systems. First, unlike bacterial expression systems, the baculovirus-based system is a eukaryotic expression system. It has the ability to produce high yields of authentically processed proteins due to the capacity of insect cells for signal peptide recognition and secretion, assembly of oligomeric proteins, proper post-translational modifications such as glycosylation, phosphorylation and disulfide bond formation. In addition, the baculovirus expression system uses a helper-independent virus that can be propagated to high titers in insect cells adapted for growth in suspension cultures, making it possible to obtain large amounts of recombinant protein with relative ease. Furthermore, the viral genome is large and thus can accommodate large segments of foreign DNA. Finally, baculoviruses are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells, which gives them a possible advantage over other systems when expressing oncogenes or potentially toxic proteins.

Except for routine production of recombinant proteins, the BES is also an effective tool for research as well as various applications in the field of biotechnology. It has shown tremendous potential as preventive, diagnostic and therapeutic agent against a myriad of diseases in the form of vaccination, tissue engineering, stem cell transduction, viral vector production and gene therapy. The BES also has been extensively used for functional studies, crystallography, biosensors, protein microarray and drug discovery.

Creative Biogene, a leading biotechnology service vendor in the world, has years of professional and abundant experience which are available to provide you a full spectrum of high quality baculovirus services ranging from baculovirus transfer vector construction, recombinant bacmid DNA preparation, baculovirus production as well as baculovirus titer assay. Creative Biogene is a reliable partner that can work with you to accelerate the speed to achieve your research goals.

References:

Murphy CI, et al. (2004). Overview of the baculovirus expression system. Current protocols in molecular biology, 65(1), 16-9.
Hitchman RB, et al. (2009). Baculovirus expression systems for recombinant protein production in insect cells. Recent patents on biotechnology, 3(1), 46-54.
Kumar N, et al. (2018). Preventive, Diagnostic and Therapeutic Applications of Baculovirus Expression Vector System. Trends in Insect Molecular Biology and Biotechnology, 163-191.
Ghosh S, et al. (2002). Baculovirus as mammalian cell expression vector for gene therapy: an emerging strategy. Molecular Therapy, 6(1), 5-11.

* For research use only. Not intended for any clinical use.

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