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GFP

Official Full Name
Green Fluorescent Protein
Background
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Synonyms
GFP; Green Fluorescent Protein

Cat.No. Product Name Price
CSC-RR0040GFP Reporter Stable Cell Line-HEK293Inquiry
CSC-RR0043GFP-Puro Reporter Stable Cell Line-HEK293TInquiry
CSC-RR0044GFP Reporter Stable Cell Line-A549Inquiry
CSC-RR0046GFP Reporter Stable Cell Line-ES-2Inquiry
CSC-RR0047GFP Reporter Stable Cell Line-HeLaInquiry
CSC-RR0048GFP Reporter Stable Cell Line-MCF-7Inquiry
CSC-RR0057GFP Reporter Stable Cell Line-NIH3T3Inquiry
CSC-RR0058GFP Reporter Stable Cell Line-OVCA429Inquiry
CSC-RR0063GFP Reporter Stable Cell Line-T47DInquiry
CSC-RR0064GFP Reporter Stable Cell Line-ZR-75-1Inquiry
CSC-RR0065GFP Reporter Stable Cell Line-HEK293Inquiry
CSC-RR0215GFP Reporter Cell Line-NCI-H1299Inquiry
CSC-RR0216GFP Reporter Cell Line-NCI-H661Inquiry
CSC-RR0217GFP Reporter Cell Line-NCI-H1975Inquiry
CSC-RR0218GFP Reporter Cell Line-AGSInquiry
CSC-RR0219GFP Reporter Cell Line-HCC70Inquiry
CSC-RR0248GFP Reporter Cell Line-A375Inquiry
CSC-RR0271GFP Reporter Cell Line-HT1080Inquiry
CSC-RR0280GFP Reporter Cell Line - SKBR3Inquiry
CSC-RR0281GFP Reporter Cell Line - COS-7Inquiry
CSC-RR0282GFP Reporter Cell Line - JurkatInquiry
CSC-RR0283GFP Reporter Cell Line - LS 174Inquiry
CSC-RR0284GFP Reporter Cell Line - PC-12Inquiry
CSC-RR0285GFP Reporter Cell Line - SK-LU-1Inquiry
CSC-RR0319GFP Reporter Cell Line-4T1.2Inquiry
CSC-RR0323POU5F1-GFP Reporter Cell Line-H9 hESCInquiry
CSC-RR0324TH-GFP Reporter Cell Line-H9 hESCInquiry
CSC-RR0325TNNT2-GFP Reporter Cell Line-H9 hESCInquiry
CSC-RR0326BEST1-GFP Reporter Cell Line-H9 hESCInquiry
CSC-RR0327GFP-ASGR1 Reporter Cell Line-H9 hESCInquiry
CSC-RR0330GFP Reporter Cell Line - B16 BL6Inquiry
CSC-RR0331GFP Reporter Cell Line - BPH-1Inquiry
CSC-RR0332GFP Reporter Cell Line - CAL-120Inquiry
CSC-RR0333GFP Reporter Cell Line - CAL-51Inquiry
CSC-RR0334GFP Reporter Cell Line - DV-90Inquiry
CSC-RR0335GFP Reporter Cell Line - HacatInquiry
CSC-RR0336GFP Reporter Cell Line - HCC78Inquiry
CSC-RR0337GFP Reporter Cell Line - HDQ-P1Inquiry
CSC-RR0338GFP Reporter Cell Line - HHUAInquiry
CSC-RR0339GFP Reporter Cell Line - HPAF-IIInquiry
CSC-RR0340GFP Reporter Cell Line - Huh7Inquiry
CSC-RR0341GFP Reporter Cell Line - KU-19-19Inquiry
CSC-RR0342GFP Reporter Cell Line - KYSE-150Inquiry
CSC-RR0343GFP Reporter Cell Line - KYSE-180Inquiry
CSC-RR0344GFP Reporter Cell Line - KYSE-520Inquiry
CSC-RR0345GFP Reporter Cell Line - LU65Inquiry
CSC-RR0346GFP Reporter Cell Line - MKN45Inquiry
CSC-RR0347GFP Reporter Cell Line - MKN74Inquiry
CSC-RR0348GFP Reporter Cell Line - OC 316Inquiry
CSC-RR0349GFP Reporter Cell Line - PA-TU-8902Inquiry
CSC-RR0350GFP Reporter Cell Line - PA-TU-8988SInquiry
CSC-RR0351GFP Reporter Cell Line - PA-TU-8988TInquiry
CSC-RR0352GFP Reporter Cell Line - RT-112Inquiry
CSC-RR0353GFP Reporter Cell Line - SK-MEL-30Inquiry
CSC-RR0354GFP Reporter Cell Line - SNB-19Inquiry
CSC-RR0359GFP Reporter Cell Line - LL/2Inquiry
CSC-RR0363GFP Reporter Cell Line - Hepa1-6Inquiry
CSC-RR0367GFP Reporter Cell Line - C1498Inquiry
CSC-RR0410GFP Labelled Human Mesenchymal Stem CellInquiry
CSC-RR0411GFP Labelled Mouse (Balb/c) Mesenchymal Stem CellInquiry
CSC-RR0412GFP Labelled Mouse (C57BL/6) Mesenchymal Stem CellInquiry
CSC-RR0413GFP Labelled Rat (F344) Mesenchymal Stem CellInquiry
CSC-RR0414GFP Labelled Rat (SD) Mesenchymal Stem CellInquiry
CSC-RR0415GFP Labelled Dog Mesenchymal Stem CellInquiry
CSC-RR0416GFP Labelled Mouse (C57BL/6) Adipose-Derived Mesenchymal Stem CellInquiry
CSC-RR0420GFP Labelled Human Umbilical Cord Mesenchymal Stem CellInquiry
CSC-RR0421GFP Labelled Human Umbilical Cord Blood Mesenchymal Stem CellInquiry
CSC-RR0424GFP Labelled Mouse (129) Embryonic Stem CellInquiry
CSC-RR0425GFP Labelled Mouse (C57BL/6) Embryonic Stem CellInquiry
CSC-RR0426GFP Labelled Mouse (C57BL/6) Neural Stem CellInquiry
CSC-RR0453HIV-1 GFP Reporter Cell Line - A3R5Inquiry
CSC-RR0456HIV-1 GFP Reporter Cell Line - CEMInquiry
CSC-RR0466GFP Reporter Cell Line - CHO-SInquiry
CSC-RR0470GFP Reporter Cell Line - K562Inquiry
CSC-RR0485GFP Reporter Cell Line - LOVOInquiry
Cat.No. Product Name Price
AAV00022ZGFP Adeno-associated virus(AAV Serotype 1)Inquiry
AAV00025ZGFP Adeno-associated virus(AAV Serotype 2)Inquiry
AAV00029ZGFP Adeno-associated virus(AAV Serotype 5)Inquiry
AAV00033ZGFP Adeno-associated virus(AAV Serotype 6)Inquiry
AAV00038ZGFP Adeno-associated virus(AAV Serotype 8)Inquiry
AAV00041ZGFP Adeno-associated virus(AAV Serotype 9)Inquiry
AAV00049ZCAG-GFP Adeno-associated virus(AAV Serotype 1)Inquiry
AAV00051ZCAG-GFP Adeno-associated virus(AAV Serotype 2)Inquiry
AAV00053ZCAG-GFP Adeno-associated virus(AAV Serotype 5)Inquiry
AAV00055ZCAG-GFP Adeno-associated virus(AAV Serotype 6)Inquiry
AAV00057ZCAG-GFP Adeno-associated virus(AAV Serotype 8)Inquiry
AAV00059ZCAG-GFP Adeno-associated virus(AAV Serotype 9)Inquiry
AAV00061ZCre-GFP Adeno-associated virus(AAV Serotype 1)Inquiry
AAV00062ZCre-GFP Adeno-associated virus(AAV Serotype 2)Inquiry
AAV00063ZCre-GFP Adeno-associated virus(AAV Serotype 5)Inquiry
AAV00064ZCre-GFP Adeno-associated virus(AAV Serotype 6)Inquiry
AAV00065ZCre-GFP Adeno-associated virus(AAV Serotype 8)Inquiry
AAV00066ZCre-GFP Adeno-associated virus(AAV Serotype 9)Inquiry
AD00003ZGFP adenoviral particlesInquiry
AD00281ZHuman GFP adenoviral particlesInquiry
AD00294ZHuman Cre-GFP adenoviral particlesInquiry
AD00323ZmMKOS-GFP adenovirusInquiry
AD00327ZcRel-GFP adenovirusInquiry
AD00384ZU6-shRNA-GFP adenovirusInquiry
AD00385ZU6-shRNA-RFP adenovirusInquiry
AD00400ZU6-Blank-GFP adenovirusInquiry
AD00401ZU6-Blank-RFP adenovirusInquiry
LV00926ZCMV-GFP lentiviral particlesInquiry
LV00938ZEF1A-GFP lentiviral particlesInquiry
LV00954LInducible tet-on GFP lentivirusInquiry
LV00958ZCAG-GFP LentivirusInquiry
LV00959ZSynapsin-GFP LentivirusInquiry
LV00965ZCMV-Cre-GFP-Puro LentivirusInquiry
LV00975ZHB9-GFP LentivirusInquiry
LV00988ZTRE-GFP LentivirusInquiry
LV00990ZSyn-GFP Lentivirus (Cre Dependent)Inquiry
LV00991Lheat inducible GFP lentivirusInquiry
LV00992LInducible tet-on GFP lentivirusInquiry
LV00993LOct4-GFP lentivirusInquiry
LV00996LPGK-GFP lentivirusInquiry
LV01004LOct4-GFP lentivirusInquiry

Green fluorescent protein (GFP) has changed from a nearly unknown protein to a commonly used molecular imaging tool in biology, genetics, medicine, and chemistry. GFP can be introduced into mammalian cells or whole organisms either by traditional plasmid transfection or viral infection. As the GFP gene is relatively small, it can be efficiently integrated into expression vectors without substantially increasing the vector's size. This feature allows vectors to be constructed that contain both a gene of interest, for instance, a therapeutic gene, and the GFP gene for use as a marker, without losing infection or transfection efficiencies. In many cases, the use of GFPs as a marker for efficient integration provides an improvement over more traditional antibiotic selection. Successfully transfected cells can be quickly sorted for GFP fluorescence by flow cytometry for immediate therapeutic use.

GFP can also be used to mark successful transgenics, especially because it is not toxic to the transgenic animal and can be monitored quickly and noninvasively by illumination by near-UV or blue light. GFP can be fused to a gene or tissue-specific promoter. And in transgenic animals, the expression can be monitored when a given tissue is subjected to certain promoter-responsive stimuli. Because GFPs can be viewed noninvasively, they may prove valuable tools for detecting tumors or diseased tissues in whole animals, or in human patients.

Based on years of experience and in-depth investigation, Creative Biogene has established a large number of GFP premade virus particles and stable GFP reporter cell lines. The GFP gene is stably integrated into the target cell genome to ensure stable reporter gene expression during continued passaging in culture. Importantly, an antibiotic selection gene (either puromycin or neomycin) is coupled to the reporter gene of interest to ensure that the cells express high levels of the desired reporter gene. Our stable GFP reporter cell lines are perfectly suited for in vivo imaging studies, where the high reporter gene expression facilitates detection of fewer implanted cells.

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