Description
RNA-guided RNA-targeting Cas13a from Leptotrichia wadei or L. shahii. Cleaves ssRNA and induces collateral cleavage upon activation.
Activity Assay
ssRNA cleavage validated
Applications
RNA editing, knockdown, and SHERLOCK detection systems
Buffer Composition
20 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 10% glycerol
Source Species
Leptotrichia sp.