Knockout Detection Kit provides a simple and highly effective method to detect gene knockout and gene polymorphism. The core component of the kit is the knockout detection enzyme, which has strong endonuclease activity and is homologous to Cel. The knockout detection enzyme can efficiently recognize and cleave the mismatches in heterogeneous DNA duplexes. Our knockout detection kit can be widely used for gene knockout detection in mammals, bacteria, fungi, virus and plants, especially applicable to screen positive clones and detect knockout efficiency using CRISPR/Cas9, TALENs or ZENs technologies.
The Knockout Detection Kit can be applied for knockout detecting of many kinds of nucleases, such as CRISPR/Cas9, TALEN and ZFN and so on.
5-20 min of digestion time is enough to detect any type of mutations such as substitution, insertion and deletion.
Enzyme digestion of PCR product from cell pool can be used to calculate mutation efficiency.
The detecting sensitivity of the Knockout Detection Kit is higher than traditional mismatch-specific DNA endonuclease based detecting method.