The cell lysate is obtained from the homozygous knockout cell line developed by CRISPR/Cas9.
Validate the specificity of antibodies.As a negative control for functional assay.
Gene knockout cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The Gene knockout cell lines are developed by CRISPR. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control.
KO cell lysate (100ug) and parental cell lysate (100ug)