CRISPR/Cas9 KO Kit is a highly effective and accurate gene editing kit which is based on the latest artificial endonuclease CRISPR/Cas9. Compared to the traditional TALEN and ZFN technologies for gene knockout, CRISPR/Cas9 KO Kit can be more easily manipulated and exhibits the highest knockout-efficiency. CRISPR/Cas9 KO Kit is applicable to modify genes in almost all kinds of mammalian cells.
The CRISPR/Cas9 KO Kit can be applied to modify genes in almost all kinds of mammalian cells.
Two functional components are combined into one vector which offers a higher transfection efficiency.
The CRISPR/Cas9 KO Kit don’t need specific species cell line. Applying eukaryotic expression system, the CRISPR/Cas9 KO Kit can be used for sgRNA activity detection and gene targeting in all mammals and relevant cell line. For non-mammals, the sgRNAs and targeting sequence can be cloned into the vectors of the kit for activity detection.
Linearized vector is provided and the DNA fragment encoding crRNA is less than 100 bp, which makes manipulation simpler. PCR product can be directly used for enzyme digestion without purification.
Hundreds of sgRNAs can be detected at one time.