Tth111 Reverse Transcriptase

Name: Tth111 Reverse Transcriptase
SKU: EMTT4005
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMTT4005

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Product Information

Cat. No.	EMTT4005
Description	Catalyzes the reverse transcriptation of RNA to cDNA at elevated temperatures in the presence of MnCl2
Source	Thermus thermophilus
Applications	Suitable for high temperature synthesis of DNA. Synthesizes cDNA from RNA template. Results in greater specificity of primer hybridization and extension of RNA. Can reverse transcribe at elevated temperatures. Minimizes problems with strong secondary structure of RNA. Used for efficient PCR of DNA, containing problematic secondary structures. Applicable to RT-PCR; the same enzyme is used for both reverse transcription and following amplification of obtained cDNA template. Resistant to amplification inhibitors present in template DNA isolated from problematic samples.
Size	500 Units;100 Units
Unit Definition	One unit is the amount of enzyme required to incorporate 1.0 nmole TTP into acid insoluble material at 50 °C in 10 minutes.
Reaction Buffer	40 mM Tris-HCl (pH 8.5) 1 mM MnCl210 mM dithiothreitol1 mg/ml bovine serum albumin0.4mM polyA(dT)0.5 mM (α-32P)TTP @ 10 μCi/ml Incubation is for 10 minutes at 50 °C in a total reaction volume of 50 μl
Reaction Conditions	Incubation at 50 °C
Storage	50 mM Tris-HCl (pH 7.5) 0.1 mM EDTA5.0 mM dithiothreitol50% (v/v) glycerol Stabilizers

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Customer Reviews

Customer Q&As

Can the cDNA obtained from this enzyme be used for RT-PCR?

A: Yes, the product obtained from this enzyme can be used for RT-PCR.

What are the reaction conditions for reverse transcription?

A: The reaction conditions for reverse transcription are incubating the reaction mixture at 50°C for 10 minutes.

What is the quality of the Tth111 Reverse Transcriptase?

A: The product has good quality with high purity, and no quality issues have been observed during its usage.

Does this enzyme require metal ions for the reverse transcription reaction?

A: Metal ions are required for the reverse transcription reaction, but they do not need to be added separately in the experiment.

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Customer Reviews

High-temperature reverse transcription

The enzyme can perform reverse transcription reactions at high temperatures, minimizing the problem of strong RNA secondary structures.

France

2021-08-15

Fast reaction speed

The reverse transcription reaction (in a reaction volume of 50 µl) can be completed by incubating at 50°C for 10 minutes.

United States

2021-10-09

Primer hybridization

Primer hybridization and RNA extension show higher specificity.

United States

2022-01-27

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Tth111 Reverse Transcriptase

Product Information

Q & A

Customer Reviews

Can the cDNA obtained from this enzyme be used for RT-PCR?

What are the reaction conditions for reverse transcription?

What is the quality of the Tth111 Reverse Transcriptase?

Does this enzyme require metal ions for the reverse transcription reaction?

High-temperature reverse transcription

Fast reaction speed

Primer hybridization

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