Creative Biogene is a biotechnology company which has the expertise to provide you with customer LAM PCR service to analyze the viral vector insertion/integration site. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to assist you in all process that LAM PCR service needed. Besides, with the help of highly experienced staffs, Creative Biogene can provide the fastest turnaround time of any supplier in the industry.
LAM - PCR (Linear - amplification mediated PCR) is a technology which used for identifying and characterizing unknown flanking DNA adjacent to known DNA of any origin. More specifically, LAM-PCR has been developed to localize viral vector integration sites (IS) within the host genome [1, 2]. Genetic elements like retroviruses or transposons integrate their genome into the host genome in a (semi-) random manner [3-6]. In many cases it is decisive to know exactly the position where these vectors integrated. Customers now can use Creative Biogene’s most affordable, high-quality LAM PCR services to solve this problem.
Creative Biogene offers custom LAM PCR services for your scientific research as follows:
LAM PCR Service
 Schmidt M, Hoffmann G, Wissler M, et al. Detection and direct genomic sequencing of multiple rare unknown flanking DNA in highly complex samples. Human gene therapy, 2001, 12(7): 743-749.
 Schmidt M, Schwarzwaelder K, Bartholomae C, et al. High-resolution insertion-site analysis by linear amplification–mediated PCR (LAM-PCR). Nature methods, 2007, 4(12): 1051-1057.
 Harkey MA, Kaul R, Jacobs MA, et al. Multiarm High-Throughput Integration Site Detection: Limitations of LAM-PCR Technology and Optimization for Clonal Analysis. Stem Cells Dev. 2007, 16(3):381-92.
 Schr?der ARW, Shinn P, Chen H, et al. HIV-1 integration in the human genome favors active genes and local hotspots. Cell, 2002, 110(4): 521-529.
 Wu X, Li Y, Crise B, et al. Transcription start regions in the human genome are favored targets for MLV integration. Science, 2003, 300(5626): 1749-1751.
 Vigdal T J, Kaufman C D, Izsvák Z, et al. Common physical properties of DNA affecting target site selection of sleeping beauty and other Tc1/mariner transposable elements. Journal of molecular biology, 2002, 323(3): 441-452.