Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : TDK-L002
| Cat. No. | TDK-L002 |
| Description | The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. |
| Features |
• Fast - 5 minutes for cohesive or blunt ends • Convenient - ligation performed at room temperature (25°C) • Flexible - suitable for all common ligation reactions |
| Applications |
• Cloning into vectors • Library construction • TA cloning • Linker ligation • Recircularization of linear DNA |
| Storage | Store at -20°C. |
A: DNA ligation is the joining of 2 DNA molecules via DNA ligase. DNA ligase catalyzes the formation of two covalent phosphodiester bonds between the 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide in an ATP-dependent reaction.
A: Up to 5 μg of blunt or dA-Tailed DNA fragments is recommended in a single Quick Ligation reaction.
A: T4 DNA ligase is an enzyme that helps create the formation of a phosphodiester bond between the 3'-hydroxyl end of a double-stranded DNA fragment and the 5'-phosphate end of the same or another DNA fragment.
A: Creative Biogene's Quick Ligation Kit enables fast sticky-end or blunt-end DNA ligation.
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Ready-to-use. Reaction mixture can be used directly for bacterial transformation.
Creative Biogene's Quick Ligation Kit allows rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro. I like it.
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