Pages
Products

Quick Ligation Kit

For research use only. Not intended for any clinical use.
Cat.No.
TDK-L002
Description
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature.
Applications
• Cloning into vectors

• Library construction

• TA cloning

• Linker ligation

• Recircularization of linear DNA
Features
• Fast - 5 minutes for cohesive or blunt ends
• Convenient - ligation performed at room temperature (25°C)
• Flexible - suitable for all common ligation reactions
Storage
Store at -20°C.

Publications

Q & A

Customer Reviews

Customer Q&As
What is DNA Ligation?

A: DNA ligation is the joining of 2 DNA molecules via DNA ligase. DNA ligase catalyzes the formation of two covalent phosphodiester bonds between the 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide in an ATP-dependent reaction.

How much DNA can I blunt in one Quick Ligation Reaction?

A: Up to 5 μg of blunt or dA-Tailed DNA fragments is recommended in a single Quick Ligation reaction.

What is T4 DNA ligase?

A: T4 DNA ligase is an enzyme that helps create the formation of a phosphodiester bond between the 3'-hydroxyl end of a double-stranded DNA fragment and the 5'-phosphate end of the same or another DNA fragment.

What type of sample prep can I use the Quick Ligation Kit for?

A: Creative Biogene's Quick Ligation Kit enables fast sticky-end or blunt-end DNA ligation.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Ready-to-use

Ready-to-use. Reaction mixture can be used directly for bacterial transformation.

United States

01/27/2022

Invaluable tool

Creative Biogene's Quick Ligation Kit allows rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro. I like it.

United States

07/19/2022

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Quick Inquiry

Inquiry