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Quick Ligation Kit

This kit contains T7 Endonuclease I and buffer. T7 Endonuclease I recognizes and cleaves mismatch–specific DNA, even single base pair mismatches, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch.
Determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I, the most application is the Crispr/cas9 monitor test.
Store at -20°C.
For research use only. Not intended for any clinical use.

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