RT-PCR (Reverse transcription-PCR) is a PCR process that uses reverse transcriptase to make DNA (cDNA) from an RNA template. The cDNA then undergoes "normal" PCR processes, under the control of DNA polymerases, resulting in the synthesis of millions of double-stranded DNA molecules representing the sequence of the original RNA.
RT-PCR is used to detect and quantify messenger RNA (mRNA). It is also used to make cDNA libraries, which can aid the study of gene expression and sequences.
Creative Biogene provides a range of RT-PCR kits for robust, convenient reverse transcription of full-length cDNA from abundant and rare mRNAs.
RT PCR Kits Products ListcDNA Synthesis Products List