microRNAs are small noncoding RNAs that serve as posttranscriptional regulators of gene expression in higher eukaryotes. They play an important role in animals and spread widely, which is highlighted by recent estimates that 20%~30% of all genes are microRNA targets. In mammalian cells, microRNAs (miRNAs) bind to the 3′ UTRs of their target mRNAs and interfere with translation. To validate these interactions between miRNAs and target gene 3’UTR region via standard methods, Creative Biogene has already created a genome-wide collection (including human, rat ,mouse, ect.) of 3’UTR which is cloned after a reporter gene. 3’UTR clones can be used to verify whether miRNA silence this gene of interest by monitoring the reporter gene expression. These plasmids can be used either for transient expession or stable expression.
Creative Biogene can provide the target gene 3’UTR clones, please browse our 3’UTR plasmid collection to find your desired one. If you don’t find the gene of your interest in our collection, we can also work for your project with a best price!3'UTR Plasmid Products List 3'UTR Virus Products List