Plasmid or cosmid DNA purified with Plasmid Endo-Free Utrapure Kit is ideal for the use in following applications:
Transfection of sensitive cells (mammalian primary/suspension cells)
Gene silencing study
Other routine cloning applications
Rapid and simple extraction
Reproducible high yields of ultrapure DNA
No toxic reagent such as ethidium bromide, phenol/chloroform, CsCl involved
Effective endotoxin removal step
Endotoxin level can be consistently less than 0. 1 EU/μg DNA in the final DN
Plasmid Endo-Free Utrapure Kit is composed of macroporous silica–based resin with a high density of diethylaminoethyl (DEAE) group, which is developed exclusively for isolation of nucleic acids. Purification is based on the interaction between negatively charged phosphates of nucleic acid backbones and positively charged DEAE groups on the surface of the resin. An innovative endotoxin-removal reagent, E2 reagent, can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the E2 reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. By testing with the endotoxin LAL test kit, the sytem consistently keeps the endotoxin level less than 0. 1 EU/μg DNA in the final plasmid DNA product.
Nucleic Acid Type
Endo-Free ultrapure DNA
For Research only.
All the kit components are guaranteed for at least 12 months from the date of purchase.