Plasmid Endo-Free Utrapure Kit

Name: Plasmid Endo-Free Utrapure Kit
SKU: NKP-T003
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : NKP-T003

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Product Information

Cat. No.	NKP-T003
Sample Type	Bacteria
Report Gene	Bacteria
Features	· Rapid and simple extraction · Reproducible high yields of ultrapure DNA · No toxic reagent such as ethidium bromide, phenol/chloroform, CsCl involved · Effective endotoxin removal step · Endotoxin level can be consistently less than 0. 1 EU/μg DNA in the final DN
Applications	Plasmid or cosmid DNA purified with Plasmid Endo-Free Utrapure Kit is ideal for the use in following applications: Transfection of sensitive cells (mammalian primary/suspension cells) Gene silencing study Microinjection Other routine cloning applications
Nucleic Acid Type	Plasmid DNA
Promoter	Gravity flow
Selection Marker	Plasmid DNA
Yield	More than 100μg
Quality	Endo-Free ultrapure DNA
Regulatory Statement	For Research only.
Note	Plasmid Endo-Free Utrapure Kit is composed of macroporous silica–based resin with a high density of diethylaminoethyl (DEAE) group, which is developed exclusively for isolation of nucleic acids. Purification is based on the interaction between negatively charged phosphates of nucleic acid backbones and positively charged DEAE groups on the surface of the resin. An innovative endotoxin-removal reagent, E2 reagent, can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the E2 reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. By testing with the endotoxin LAL test kit, the sytem consistently keeps the endotoxin level less than 0. 1 EU/μg DNA in the final plasmid DNA product.
Storage	All the kit components are guaranteed for at least 12 months from the date of purchase.

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