Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity.
First-Strand cDNA Synthesis
1× M-MuLV Reverse Transcriptase Reaction System containing: 1× M-MuLV Reverse Transcriptase Reaction Buffer [50 mM Tris-HCl (pH 8.3 at 25°C), 75 mM KCl, 3mM MgCl2, 10 mM DTT]; add dNTPs (not supplied with enzyme); incubate at 37°C or 42°C.
10,000 U; 50,000 U
A recombinant E. coli strain that carries the cloned M-MLV Reverse Transcriptase gene.
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly (A): oligo (dT) as template-primer.