M-MLV Reverse Transcriptase

Name: M-MLV Reverse Transcriptase
SKU: EMQZ1406
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1406

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Product Information

Cat. No.	EMQZ1406
Description	Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity.
Source	A recombinant E. coli strain that carries the cloned M-MLV Reverse Transcriptase gene.
Concentration	200,000 U/ml
Applications	First-Strand cDNA Synthesis
Size	10,000 U; 50,000 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly (A): oligo (dT) as template-primer.
Reaction Conditions	1× M-MuLV Reverse Transcriptase Reaction System containing: 1× M-MuLV Reverse Transcriptase Reaction Buffer [50 mM Tris-HCl (pH 8.3 at 25°C), 75 mM KCl, 3mM MgCl2, 10 mM DTT]; add dNTPs (not supplied with enzyme); incubate at 37°C or 42°C.
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

When reverse transcribing, can the template only be extracted RNA?

A: No, this product can be used to synthesize cDNA using RNA as the template, and it can also be used to synthesize a complementary DNA strand using single-stranded DNA as the template.

Is it necessary to perform reverse transcription on ice?

A: No, it is not necessary to perform reverse transcription on ice. The enzyme is relatively stable and can be added to the reaction system directly at room temperature.

Can the product be stored at 4℃ after reverse transcription is complete?

A: It is not recommended. After reverse transcription is complete, the product is DNA, and it is best to store it at -20℃ or lower. It can be stored briefly (no more than 1 day) at 4℃.

Does the enzyme have 3'→5' exonuclease activity?

A: No, M-MLV reverse transcriptase lacks 3'→5' exonuclease activity.

Can this enzyme be used for templates with high GC content?

A: Yes, this enzyme has good thermal stability. If the template has a high GC content or complex secondary structure, the reverse transcription temperature can be increased to 55℃-60℃.

Is the success rate of the experiment high?

A: As long as the instructions (such as the reaction system) are followed, the success rate of the experiment is generally high.

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Customer Reviews

Suitable for long fragment reverse transcription

Our laboratory successfully detected the RNA level of a certain protein in mice (>5kb) using this enzyme.

United States

2022-06-16

Simple and fast reverse program

Only two steps are needed, and after the reverse transcription at 37℃/42℃, the enzyme is inactivated by heating at 70℃ for 10 minutes.

United States

2023-01-12

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M-MLV Reverse Transcriptase

Product Information

Q & A

Customer Reviews

When reverse transcribing, can the template only be extracted RNA?

Is it necessary to perform reverse transcription on ice?

Can the product be stored at 4℃ after reverse transcription is complete?

Does the enzyme have 3'→5' exonuclease activity?

Can this enzyme be used for templates with high GC content?

Is the success rate of the experiment high?

Suitable for long fragment reverse transcription

Simple and fast reverse program

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