Luc Reporter Cell Line-HCT116 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and puromycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, HCT116 cells can form tumors in mouse. The in vivo growth of these tumors can be monitored using bioluminescent imaging.