Luc Reporter Cell Line-CT26 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and neomycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, CT26 cells can form tumors post implantation into mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging.