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Klenow Fragment (3′→5′exo-)

Cat.No.
EMQZ1421
Description
Klenow fragment (3' to 5' exo-) is proteolytic product of E. coli DNA Polymerase I which retains polymerization and 5' to 3' exonuclease activity, but has lost 3' to 5' exonuclease activity with D355A and E357A mutation.
Source Description
A recombinant E.coli strain that carries polA gene fragment with D355A and E357A mutation.
Applications
• DNA sequencing by the Sanger dideoxy method • Second strand synthesis of cDNA; • Synthesis of random primers
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 37°C.
Reaction Conditions
1×Klenow fragment (3' to 5' exo-) Reaction Buffer: [10 mM Tris-HCl (pH 7.9 at 25°C),50 mM NaCl,10 mM MgCl2,1 mM DTT]; add dNTPs (not supplied with enzyme),incubate at 37°C.
Concentration
5,000 U/ml
Size
200 U; 1,000 U
Storage Conditions
Store at -20°C

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Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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