Human TAD Kinase Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa

Name: Human TAD Kinase Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa
SKU: CSC-RR0036
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0036

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0036
Introduction	Phosphorylation of proteins, catalyzed by kinases, is critical to cell functions involved with signal transduction. This process involves a change in the phosphorylation status of downstream proteins by modification of specific serine/threonine or tyrosine residues. Inappropriate or deregulated phosphorylation is often associated with diseases, such as diabetes, cancer, heart disease, and neurological and autoimmune diseases. A clear understanding of phosphorylation associated with a specific signaling pathway is the key to understanding pathway activation, related diseases and ultimate treatment. Many end proteins of signal transduction are transcription factors (TFs). TFs usually contain two functional domains, a DNA binding domain (DBD), for interaction with promoters of genes, and a trans-activation domain (TAD), for modulation of the activity of transcriptional machinery. The activity of TADs is often regulated by kinases, with different TADs being modified by specific kinases. Therefore, these domains become valuable targets for the study of kinases, phosphorylation, signal transduction, and transcriptional activation.
Target Gene	TAD
Gene Species	Homo sapiens (Human)
Abbr	HeLa-HuTAD Kinase Prom/Luciferase/Hyg
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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